Cytotypically Specific Transfecting Activity of DNA from C57BL/Ka Mouse Cells Producing Thymotropic in Contrast to Nonthymotropic Retroviruses

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 77; no. 10; pp. 6189 - 6193
• Main Authors: Kopecka, Helena, Declève, Alain, Lieberman, Miriam, Fry, Kirk, Kaplan, Henry S.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 01-10-1980; National Acad Sciences
• Subjects: Animals; Cell culture techniques; Cell lines; Cellular immunity; Cultured cells; DNA; DNA, Viral - genetics; Fibroblasts; Genotypes; Leukemia Virus, Murine - genetics; Lymphoma; Mice; Mice, Inbred Strains - microbiology; Species Specificity; Thymus Gland - microbiology; Transfection; Virus Replication; Viruses
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.77.10.6189

A comparative study has been made of the susceptibility of fibroblastic cells to transfection by DNA from C57BL/Ka mouse lines producing either fibrotropic or thymotropic retroviruses. DNA isolated from fibroblasts that release a B-ecotropic, fibrotropic virus, BL/Ka(B), was found to transfect fibroblasts of Fv-1bb genotype with release of virus similar to BL/Ka(B). Fv-1nn fibroblasts were also susceptible but expression was delayed, and xenotypic mink lung cells were refractory. In contrast, DNA prepared from a murine T-cell lymphoma line producing a B-ecotropic, thymotropic virus failed to transfect mouse fibroblasts though it transfected a nonproducer T-cell lymphoma line. The data suggest that the Fv-1 and differentiation-specific restriction mechanisms operate at different molecular levels.