High-performance liquid chromatography with diode array detection coupled to electrospray time-of-flight and ion-trap tandem mass spectrometry to identify phenolic compounds from a lemon verbena extract

• Published in: Journal of Chromatography A Vol. 1216; no. 28; pp. 5391 - 5397
• Main Authors: Quirantes-Piné, R., Funes, L., Micol, V., Segura-Carretero, A., Fernández-Gutiérrez, A.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 10-07-2009; Amsterdam; New York: Elsevier; Elsevier
• Subjects: Aloysia citrodora; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; General pharmacology; Glucosides - isolation & purification; High-performance liquid chromatography; Lemon verbena; Mass spectrometry; Medical sciences; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Phenolic compounds; Phenols - isolation & purification; plant extracts; Plant Extracts - isolation & purification; Spectrometry, Mass, Electrospray Ionization - methods; Tandem Mass Spectrometry - methods; Verbena - chemistry; Lemon verbena; Phenolic compounds; High-performance liquid chromatography; Mass spectrometry; Iridoid; Tandem mass spectrometry; Chemical analysis; Metabolite; HPLC chromatography; Diphenols; Flavonoid; Disaccharide; Electrospray; Glucuroconjugate; Medicinal plant; Ion trap; Dicotyledones; Angiospermae; Diode array; Glycoside; Quantitative analysis; Terpenoid; Coupled method; Pharmacognosy; Reversed phase chromatography; Ultraviolet detector; Verbenaceae; Time of flight method; Plant origin; Uronic acid; Flavone derivatives; Phenols; Aloysia triphylla; Monoterpene; Spermatophyta
• ISSN: 0021-9673; 1873-3778
• DOI: 10.1016/j.chroma.2009.05.038

High-performance liquid chromatography with diode array and electrospray ionization mass spectrometric detection was used to carry out the comprehensive characterization of a lemon verbena extract with demonstrated antioxidant and antiinflammatory activity. Two different MS techniques have been coupled to HPLC: on one hand, time-of-flight mass spectrometry, and on the other hand, tandem mass spectrometry on an ion-trap. The use of a small particle size C18 column (1.8
μm) provided a great resolution and made possible the separation of several isomers. The UV–visible spectrophotometry was used to delimit the class of phenolic compound and the accurate mass measurements on time-of-flight spectrometer enabled to identify the compounds present in the extract. Finally, the fragmentation pattern obtained in MS–MS experiments confirmed the proposed structures. This procedure was able to determine many well-known phenolic compounds present in lemon verbena such as verbascoside and its derivatives, diglucuronide derivatives of apigenin and luteolin, and eukovoside. Also gardoside, verbasoside, cistanoside F, theveside, campneoside I, chrysoeriol-7-diglucuronide, forsythoside A and acacetin-7-diglucuronide were found for the first time in lemon verbena.