Activation profile of CXCL8-stimulated neutrophils and aging

• Published in: Cytokine (Philadelphia, Pa.) Vol. 61; no. 3; pp. 716 - 719
• Main Authors: Dalboni, Thalita Marcato, Abe, Aneli Eiko, de Oliveira, Carine Ervolino, Lara, Vanessa Soares, Campanelli, Ana Paula, Gasparoto, Carlos Teodoro, Gasparoto, Thaís Helena
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-03-2013
• Subjects: Adult; Aged; Aggression; Aging; Aging - immunology; Antimicrobial enzymes; Cell activation; CXCL8; Cytokines; Data processing; Effector cells; Elastase; Elderly; Geriatrics; Granulocyte-macrophage colony-stimulating factor; Humans; Immunity; innate immunity; Interleukin 10; Interleukin 6; Interleukin-8 - pharmacology; Leukocytes (neutrophilic); Lipopolysaccharides; Microorganisms; Middle Aged; myeloperoxidase; Neutrophil Activation - drug effects; Neutrophils; Neutrophils - drug effects; Neutrophils - enzymology; Neutrophils - immunology; Pancreatic Elastase - metabolism; Peroxidase; Peroxidase - metabolism; receptors; recruitment; secretion; Young Adult; CXCL8; Elderly; Neutrophils; Antimicrobial enzymes
• ISSN: 1043-4666; 1096-0023
• DOI: 10.1016/j.cyto.2013.01.016

► First description about human neutrophils from elderly individuals exogenous CXCL8. ► CXCL8 activated-neutrophils from elderly individuals did not secrete IL-6. ► CXCL8 or LPS unaffected myeloperoxidase of neutrophils from elderly individuals. ► LPS-activated neutrophils from elderly individuals produced increased IL-10 level. ► LPS and CXCL8-activated neutrophils from aged individuals produced increased GM-CSF.
Neutrophils are pivotal effector cells of innate immunity representing the first line of defense against aggression. They are the first cells to arrive at the site of the aggression, where they can directly eliminate the invading microorganisms. Their activation and recruitment into peripheral tissues is indispensable for host defense. With aging, there are alterations of the receptor by driven functions of human neutrophils as a decrease in the functional changes in signaling elicited by specific receptors, as CXCR1. We investigated the activation of neutrophils from elderly after the cells were cultivated with CXCL8. Although, CXCL8 induced elastase (ELA) secretion, data showed neither myeloperoxidase (MPO) activity nor production of IL-6, IL-10, GM-CSF by neutrophils from elderly compared with young individuals. On the other hand, in the presence of only LPS or LPS associated with CXCL8 neutrophils from elderly individuals, there were significant levels of IL-6, IL-10, GM-CSF but not MPO. These results indicate that neutrophils from elderly do not respond to CXCL8 stimulus, but they are activated by LPS to produce cytokines. However, MPO activity from elderly individuals was not different in the presence or absence of LPS and CXCL8.