Characterization of (Na +, K +)-ATPase in gill microsomes of the freshwater shrimp Macrobrachium olfersii

To better understand the adaptive strategies that led to freshwater invasion by hyper-regulating Crustacea, we prepared a microsomal (Na +, K +)-ATPase by differential centrifugation of a gill homogenate from the freshwater shrimp Macrobrachium olfersii. Sucrose gradient centrifugation revealed a li...

Full description

Saved in:

Bibliographic Details
Published in:	Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology Vol. 126; no. 3; pp. 303 - 315
Main Authors:	Furriel, R.P.M., McNamara, J.C., Leone, F.A.
Format:	Journal Article
Language:	English
Published:	England Elsevier Inc 01-07-2000
Subjects:	(Na+, K+)-ATPase Animals ATP Crustacean gill Enzyme Activation Freshwater shrimp Gills - enzymology Gills - ultrastructure Macrobrachium Microsomal fraction Microsomes - enzymology Ouabain Palaemonidae - enzymology Palaemonidae - ultrastructure Sodium-Potassium-Exchanging ATPase - analysis Vanadate BCIP, 5-bromo-4-chloro-3-indole Ouabain LDH, lactate dehydrogenase AMPOL, 2-amino-2-methylpropan-1-ol PGK, phosphoglycerate kinase Vanadate DMSO, dimethylsulfoxide (Na+, K+)-ATPase Freshwater shrimp NBT, nitroblue tetrazolium Macrobrachium PK, pyruvate kinase Microsomal fraction PEP, phosphoenolpyruvate ATP Crustacean gill GAPDH, glyceraldehyde-3-phosphate dehydrogenase
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Be the first to leave a comment!