Quantitative trait loci analysis of tail tendon break time in mice of C57BL/6J and DBA/2J lineage

• Published in: The journals of gerontology. Series A, Biological sciences and medical sciences Vol. 66; no. 2; pp. 170 - 178
• Main Authors: Sloane, Lauren B, Stout, Joseph T, Vandenbergh, David J, Vogler, George P, Gerhard, Glenn S, McClearn, Gerald E
• Format: Journal Article
• Language: English
• Published: United States Oxford University Press 01-02-2011
• Subjects: Age differences; Aging - genetics; Animals; Chromosome Mapping - methods; Collagen; Collagen - genetics; Female; Genes; Genetics; Journal of Gerontology: BIOLOGICAL SCIENCES; Male; Mice; Mice, Inbred C57BL; Mice, Inbred DBA; Quantitative Trait Loci; Receptor for Advanced Glycation End Products; Receptors, Immunologic - genetics; Rodents; Sex Factors; Tail; Tendons
• ISSN: 1079-5006; 1758-535X
• DOI: 10.1093/gerona/glq169

Tail tendon break time (TTBT), a measure of collagen cross-linking, shown to increase with age differs significantly among inbred strains of mice, indicating underlying genetic influences. This study was aimed to identify quantitative trait loci (QTLs) associated with tail tendon break time at three ages (200, 500, and 800 days of age) for 23 BxD recombinant inbred strains of mice and B6D2F(2) mice derived from C57BL/6J and DBA/2J strains. Heritability estimates were calculated, and QTL analyses were conducted using interval-mapping methods. Mean tail tendon break time values were higher in males and increased nonlinearly with age. Eight total QTLs were nominated in the B6D2F(2) mice at the three measured ages, with the QTL at 800 days confirmed in the recombinant inbred strains. Allelic effect modeling for the identified QTLs suggests differences in gene action between sexes. Candidate genes in the QTL regions include collagen genes and an advanced glycation end-product receptor. The QTLs identified demonstrate influence at some but not all ages.