A novel and simple heat-based method eliminates the highly detrimental effect of xylene deparaffinization on acid-fast stains

Abstract Objectives Histopathology is an important method for diagnosing extrapulmonary tuberculosis, yet tissue sections are often negative for mycobacteria after use of acid-fast stain (AFS). This study investigated the mechanism of AFS use and the detrimental effect of histologic processing—in pa...

Full description

Saved in:

Bibliographic Details
Published in:	American journal of clinical pathology Vol. 160; no. 1; pp. 81 - 88
Main Authors:	Marinho, Pedro F, Vieira, Soraia L, Carvalho, Tânia G, Peleteiro, Maria C, Hanscheid, Thomas
Format:	Journal Article
Language:	English
Published:	US Oxford University Press 05-07-2023
Subjects:	Acids BCG BCG vaccines Benzophenoneidum Cell walls Coloring Agents Diagnosis Fluorescence Histochemistry Hot Temperature Humans Localization Methods Methylene blue Mycobacterium RNA Solvents Staining and Labeling Stains & staining Tuberculosis Xylene Xylenes Portugal Acid-fast stain Histology Novel method Xylene deparaffinization Mycobacteria
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Be the first to leave a comment!