Short communication: effect of trans-10,cis-12 conjugated linoleic acid on activation of lipogenic transcription factors in bovine mammary epithelial cells

• Published in: Journal of dairy science Vol. 97; no. 8; p. 5001
• Main Authors: Ma, L, Lengi, A J, McGilliard, M L, Bauman, D E, Corl, B A
• Format: Journal Article
• Language: English
• Published: United States 2014
• Subjects: Animals; Cattle; Cell Count; Epithelial Cells - drug effects; Epithelial Cells - metabolism; Fatty Acids - metabolism; Isomerism; Linoleic Acid; Linoleic Acids, Conjugated - pharmacology; Lipogenesis; Liver X Receptors; Orphan Nuclear Receptors - genetics; Orphan Nuclear Receptors - metabolism; PPAR gamma - genetics; PPAR gamma - metabolism; Sterol Regulatory Element Binding Protein 1 - genetics; Sterol Regulatory Element Binding Protein 1 - metabolism; Transcription Factors - genetics; Transcription Factors - metabolism; transcription factor; luciferase reporter; trans-10,cis-12 conjugated linoleic acid
• ISSN: 1525-3198
• DOI: 10.3168/jds.2013-7555

The objective of this study was to examine the effect of trans-10,cis-12 conjugated linoleic acid (t10c12CLA) on the activation of transcription factors that potentially regulate lipid synthesis in a bovine mammary epithelial cell line (MAC-T). Cells were transfected with luciferase reporter constructs containing sterol response element (SRE and SRE complex) for sterol regulatory element binding protein-1, peroxisome proliferator response element for peroxisome proliferator-activated receptor γ, or liver X receptor response element for liver X receptor. Different concentrations of t10c12CLA (0, 25, 50, 75, or 100μM) were applied to cells to determine the activation of transcription factors. The influence of t10c12CLA bond structure on transcription factor activation was also investigated by treating cells with different 18:1 fatty acid isomers (trans-10 18:1 or cis-12 18:1) at 100μM. Cells were harvested for luciferase assay after 24h of treatment. Compared with linoleic acid and cis-9,trans-11 CLA controls, the SRE reporters had significantly lower activity in t10c12CLA-treated cells at 50 and 75μM for SRE complex and SRE, respectively. Lower SRE and SRE complex activation was observed in t10c12CLA treatment at 25, 50, and 75μM compared with 0μM. The peroxisome proliferator response element and liver X receptor response element reporters did not respond differently between the t10c12CLA treatment and controls. Compared with t10c12CLA, both trans-10 18:1 and cis-12 18:1 increased the activities of SRE and SRE complex reporters by 1.3- to 4.2-fold. In conclusion, t10c12CLA has an inhibitory role in lipogenic transcription factor activation of SRE, and this negative effect is due to the conjugation of trans-10 and cis-12 double bonds in the fatty acid. Furthermore, we found no support for a regulatory role of response elements for peroxisome proliferator-activated receptor γ or liver X receptor in the t10c12CLA inhibition of mammary lipid synthesis.