Biological control of Ascaris suum eggs by Pochonia chlamydosporia fungus

• Published in: Veterinary research communications Vol. 35; no. 8; pp. 553 - 558
• Main Authors: Ferreira, Sebastião Rodrigo, de Araújo, Jackson Victor, Braga, Fábio Ribeiro, Araujo, Juliana Milani, Frassy, Luiza Neme, Ferreira, Aloízio Soares
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-12-2011; Springer Nature B.V
• Subjects: Animals; Ascariasis - prevention & control; Ascariasis - veterinary; Ascaris suum - microbiology; Ascomycota - physiology; Biomedical and Life Sciences; Host-Pathogen Interactions; Life Sciences; Ovum - microbiology; Pest Control, Biological - methods; Short Communication; Swine; Swine Diseases - parasitology; Swine Diseases - prevention & control; Veterinary Medicine/Veterinary Science; Zoology; Nematophagous fungi; Biological control
• ISSN: 0165-7380; 1573-7446
• DOI: 10.1007/s11259-011-9494-6

Ascaris suum
is a gastrointestinal nematode parasite of swines. The aim of this study was to observe
Pochonia chlamydosporia
fungus on biological control of
A. suum
eggs after fungus passage through swines gastrointestinal tract. Eighteen pigs, previously dewormed, were randomly divided into three groups: group 1, treated with the fungus isolate VC4; group 2, treated with the fungus isolate VC1 and group 3 did not receive fungus (control). In the treated groups, each animal received a 9 g single dose of mycelium mass containing
P. chlamydosporia
(VC1 or VC4). Thereafter, animal fecal samples were collected at the following intervals: 8, 12, 24, 36, 48, 72 and 96 h after treatment beginning and these were poured in Petri dishes containing 2% water-agar culture medium. Then, 1,000
A. suum
eggs were poured into each dish and kept in an incubator at 26°C and in the dark for 30 days. After this period, approximately 100 eggs were removed from each Petri dish and morphologically analyzed under light microscopy following the ovicidal activity parameters. The higher percentage observed for isolated VC4 eggs destruction was 57.5% (36 h) after fungus administration and for isolate VC1 this percentage was 45.8% (24 h and 72 h) (
p
 > 0.01).
P. chlamydosporia
remained viable after passing through the gastrointestinal tract of swines, maintaining its ability of destroying
A. suum
eggs.