An evaluation of multiplex bead-based analysis of cytokines and soluble proteins in archived lithium heparin plasma, EDTA plasma and serum samples

Objective: To assess the usability of archived plasma and serum by multiplex (Luminex) analysis of circulating proteins (analytes) by evaluating the day to day variation, the effect of several freeze-thaw cycles, and the influence of the media and choice of anticoagulant. Methods: Nineteen analytes...

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Bibliographic Details
Published in:	Scandinavian journal of clinical and laboratory investigation Vol. 76; no. 8; pp. 601 - 611
Main Authors:	Brøndum, Line, Sørensen, Brita Singers, Eriksen, Jesper Grau, Mortensen, Lise Saksø, Lønbro, Simon, Overgaard, Jens, Alsner, Jan
Format:	Journal Article
Language:	English
Published:	England Taylor & Francis 16-11-2016
Subjects:	Adult Aged Aged, 80 and over Anticoagulants - chemistry Blood Case-Control Studies Cryopreservation cytokines Cytokines - blood Edetic Acid - chemistry Female Head and Neck Neoplasms - blood Heparin - chemistry Hepatocyte Growth Factor - blood Humans Immunoassay - methods Male Middle Aged multiplex analysis Observer Variation Quality Control Receptor, Epidermal Growth Factor - blood Reproducibility of Results specimen handling Specimen Handling - methods Vascular Endothelial Growth Factor A - blood Vascular Endothelial Growth Factor Receptor-1 - blood Vascular Endothelial Growth Factor Receptor-2 - blood specimen handling cytokines multiplex analysis quality control Blood
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