Pressure provides new insights into protein folding, dynamics and structure

• Published in: Trends in Biochemical Sciences Vol. 26; no. 10; pp. 612 - 618
• Main Authors: Silva, Jerson L, Foguel, Debora, Royer, Catherine A
• Format: Book Review Journal Article
• Language: English
• Published: England Elsevier Ltd 01-10-2001
• Subjects: Capsid - chemistry; folding intermediates; folding kinetics; Hydrostatic Pressure; Macromolecular Substances; Models, Molecular; NMR; protein cavities; Protein Denaturation; Protein Folding; Proteins - chemistry; Thermodynamics; NMR; folding intermediates; folding kinetics; Biochemistry; hydrostatic pressure; protein cavities; Structural biology
• ISSN: 0968-0004; 1362-4326
• DOI: 10.1016/S0968-0004(01)01949-1

Hydrostatic pressure is a powerful tool for studying protein folding, and the dynamics and structure of folding intermediates. Recently, pressure techniques have opened two important fronts to aid our understanding of how polypeptides fold into highly structured conformations. The first advance is the stabilization of folding intermediates, making it possible to characterize their structures and dynamics by different methodologies. Kinetic studies under pressure constitute the second advance, promising detailed appraisal and understanding of protein folding landscapes. The combination of these two approaches enables dissection of the roles of packing and cavities in folding, and in assembly of multimolecular structures such as protein–DNA complexes and viruses. The study of aggregates and amyloids, derived from partially folded intermediates at the junction between productive and off-pathway folding, have also been studied, promising better understanding of diseases associated with protein misfolding.