A novel huperzine A-producing endophytic fungus Fusarium sp. Rsp5.2 isolated from Huperzia serrate

• Published in: Biotechnology letters Vol. 42; no. 6; pp. 987 - 995
• Main Authors: Le, Thanh Thi Minh, Hoang, Anh Thi Hong, Nguyen, Nhue Phuong, Le, Thuy Thi Bich, Trinh, Ha Thi Thu, Vo, Thuy Thi Bich, Quyen, Dong Van
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-06-2020; Springer Nature B.V
• Subjects: Acetylcholinesterase; Alkaloids - analysis; Alkaloids - metabolism; Applied Microbiology; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cholinesterase Inhibitors - analysis; Cholinesterase Inhibitors - metabolism; Chromatography; Chromatography, High Pressure Liquid; Endophytes; Esterase; Fermentation; Fungi; Fusarium; Fusarium - cytology; Fusarium - isolation & purification; Fusarium - metabolism; High performance liquid chromatography; Huperzia - microbiology; Huperzine A; Life Sciences; Liquid chromatography; Microbiology; Mycelium - metabolism; Original Research Paper; Physical characteristics; Sesquiterpenes - analysis; Sesquiterpenes - metabolism; Thin layer chromatography; Vietnam; Vietnam; sp. Rsp5.2; Endophytic fungi; Huperzine A; Alzheimer disease; Huperzia serrata; Fusarium sp. Rsp5.2
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-020-02836-x

The isolation, identification and characterization of a novel huperzine A (HupA)-producing fungal strain Rsp5.2 isolated from
Huperzia serrata
(Thunb. ex Murray) Trev. in Vietnam. The fifty-eight endophytic fungi were recovered from roots of natural
H. serrata
in Lao Cai province of northern Vietnam and screened for HupA-producing by thin-layer chromatography (TLC). The only one of the 58 strains, Rsp5.2, could produce HupA. The amount of HupA produced by Rsp5.2 was quantified to be 19.45 µg g
−1
dried mycelium by high-performance liquid chromatography (HPLC). Acetylcholine esterase (AchE) inhibition (IC
50
) of the crude HupA extract from Rsp5.2 fermentation broth was 2.849 ± 0.0026 µg mL
−1
. The fungus was identified as
Fusarium
sp. Rsp5.2 by morphological characteristics and Internal Transcribed Spacer (ITS) sequences. This is the first report of
Fusarium
sp. as a HupA-producing endophyte isolated from
H. serrata
.