Sepsis induced by cecal ligation and perforation (CLP) alters nucleotidase activities in platelets of rats

• Published in: Microbial pathogenesis Vol. 111; pp. 345 - 351
• Main Authors: Pereira, Renata S., Bertoncheli, Claudia M., Adefegha, Stephen A., Castilhos, Lívia G., Silveira, Karine L., Rezer, João Felipe P., Doleski, Pedro H., Abdalla, Fátima H., Santos, Karen F., Leal, Claudio A.M., Santos, Roberto C.V., Casali, Emerson A., Moritz, Cesar E.J., Stainki, Daniel R., Leal, Daniela B.R.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-10-2017
• Subjects: 5'-Nucleotidase - metabolism; Adenosine Monophosphate - metabolism; Adenosine Triphosphate - metabolism; Animals; Blood Platelets - enzymology; Blood Platelets - metabolism; Cecum - surgery; E-5-Nucleotidase; E-ADA; E-NTPDase; Humans; Ligation - adverse effects; Male; Platelets; Postoperative Complications - enzymology; Postoperative Complications - etiology; Postoperative Complications - metabolism; Postoperative Complications - microbiology; Rats; Rats, Wistar; Sepsis; Sepsis - enzymology; Sepsis - etiology; Sepsis - metabolism; Sepsis - microbiology; Sepsis; E-NTPDase; E-5-Nucleotidase; Platelets; E-ADA
• ISSN: 0882-4010; 1096-1208
• DOI: 10.1016/j.micpath.2017.08.047

Sepsis is a potentially lethal condition, and it is associated with platelet alterations. The present study sought to investigate the activity of ecto-nucleoside triphosphate diphosphohydrolase (E-NTPDase), E-5′-nucleotidase, and ecto-adenosine deaminase (E-ADA) in the platelets of rats that were induced with sepsis. Male Wistar rats were divided into three groups of ten animals each: a negative control group (normal; NC); a group that underwent surgical procedures (sham); and a group that underwent cecal ligation and perforation (CLP). The induction of sepsis was confirmed by bacteremia, and the causative pathogen identified was Escherichia coli. Hematological parameters showed leukocytosis and thrombocytopenia in animals in the septic group. The results also revealed that there were significant (p < 0.05) increases in adenosine triphosphate (ATP) and adenosine monophosphate (AMP) hydrolyses, and in the deamination of adenosine in the CLP group compared to the sham and control groups. Conversely, ADP hydrolysis was significantly decreased (p < 0.05) in the CLP group compared to the sham and control groups. Purine levels were analyzed by high-performance liquid chromatography (HPLC) in serum samples from control, sham, and CLP groups. Increased concentrations of ATP, adenosine, and inosine were found in the CLP group compared to the sham and control groups. Conversely, the concentrations of ADP and AMP in the CPL group were not significantly altered. We suggest that alterations in hematological parameters, nucleotide hydrolysis in platelets, and nucleotide concentrations in serum samples of rats with induced sepsis may be related to thromboembolic events.
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•The activity of enzymes that degrade adenine nucleotides were evaluated in platelets of rats with sepsis induced.•Increase in hydrolysis of ATP, AMP and ADA and decrease of ADP in CLP group.•The alterations on hydrolysis of nucleotide may be related to thromboembolic events observed in sepsis.