Vasoactive intestinal peptide (VIP) induces malignant transformation of the human prostate epithelial cell line RWPE-1

Abstract The carcinogenic potential of vasoactive intestinal peptide (VIP) was analyzed in non-tumor human prostate epithelial cells (RWPE-1) and in vivo xenografts. VIP induced morphological changes and a migratory phenotype consistent with stimulation of expression/activity of metalloproteinases M...

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Bibliographic Details
Published in:Cancer letters Vol. 299; no. 1; pp. 11 - 21
Main Authors: Fernández-Martínez, Ana B, Bajo, Ana M, Isabel Arenas, M, Sánchez-Chapado, Manuel, Prieto, Juan C, Carmena, María J
Format: Journal Article
Language:English
Published: Ireland Elsevier Ireland Ltd 18-12-2010
Elsevier Limited
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Summary:Abstract The carcinogenic potential of vasoactive intestinal peptide (VIP) was analyzed in non-tumor human prostate epithelial cells (RWPE-1) and in vivo xenografts. VIP induced morphological changes and a migratory phenotype consistent with stimulation of expression/activity of metalloproteinases MMP-2 and MMP-9, decreased E-cadherin-mediated cell–cell adhesion, and increased cell motility. VIP increased cyclin D1 expression and cell proliferation that was blocked after VPAC1 -receptor siRNA transfection. Similar effects were seen in RWPE-1 tumors developed by subcutaneous injection of VIP-treated cells in athymic nude mice. VIP acts as a cytokine in RWPE-1 cell transformation conceivably through epithelial–mesenchymal transition (EMT), reinforcing VIP role in prostate tumorigenesis.
ISSN:0304-3835
1872-7980
DOI:10.1016/j.canlet.2010.07.019