Vasoactive intestinal peptide (VIP) induces malignant transformation of the human prostate epithelial cell line RWPE-1
Abstract The carcinogenic potential of vasoactive intestinal peptide (VIP) was analyzed in non-tumor human prostate epithelial cells (RWPE-1) and in vivo xenografts. VIP induced morphological changes and a migratory phenotype consistent with stimulation of expression/activity of metalloproteinases M...
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Published in: | Cancer letters Vol. 299; no. 1; pp. 11 - 21 |
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Main Authors: | , , , , , |
Format: | Journal Article |
Language: | English |
Published: |
Ireland
Elsevier Ireland Ltd
18-12-2010
Elsevier Limited |
Subjects: | |
Online Access: | Get full text |
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Summary: | Abstract The carcinogenic potential of vasoactive intestinal peptide (VIP) was analyzed in non-tumor human prostate epithelial cells (RWPE-1) and in vivo xenografts. VIP induced morphological changes and a migratory phenotype consistent with stimulation of expression/activity of metalloproteinases MMP-2 and MMP-9, decreased E-cadherin-mediated cell–cell adhesion, and increased cell motility. VIP increased cyclin D1 expression and cell proliferation that was blocked after VPAC1 -receptor siRNA transfection. Similar effects were seen in RWPE-1 tumors developed by subcutaneous injection of VIP-treated cells in athymic nude mice. VIP acts as a cytokine in RWPE-1 cell transformation conceivably through epithelial–mesenchymal transition (EMT), reinforcing VIP role in prostate tumorigenesis. |
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ISSN: | 0304-3835 1872-7980 |
DOI: | 10.1016/j.canlet.2010.07.019 |