Isolation of the human genomic brain-2/N-Oct 3 gene (POUF3) and assignment to chromosome 6q16

• Published in: Genomics (San Diego, Calif.) Vol. 26; no. 2; pp. 272 - 280
• Main Authors: Atanasoski, S., Toldo, S.Solinas, Malipiero, U., Schreiber, E., Fries, R., Fontana, A.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 20-03-1995; Elsevier
• Subjects: Animals; Base Sequence; Biological and medical sciences; Chromosome Mapping; Chromosomes, Human, Pair 6; Classical genetics, quantitative genetics, hybrids; Fundamental and applied biological sciences. Psychology; Genes; Genetics of eukaryotes. Biological and molecular evolution; Genome, Human; Homeodomain Proteins; Human; Humans; In Situ Hybridization, Fluorescence; Mice; Molecular Sequence Data; Nerve Tissue Proteins - genetics; POU Domain Factors; Sequence Alignment; Sequence Homology, Nucleic Acid; Species Specificity; Transcription Factors - genetics; Transcription, Genetic; Genetic mapping; Human; Transcription factor N-Oct3; Nucleotide sequence; Transcription promoter; Central nervous system; Homology; Gene organization; Gene expression; Gene; Chromosome DNA; C6-Chromosome; Brain (vertebrata)
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1016/0888-7543(95)80211-4

N-Oct 3 is a human POU domain transcription factor that binds to the octamer sequence ATGCAAAT. The protein is expressed in the central nervous system during development and in adult brain. We have isolated and characterized genomic clones encoding the human N-Oct 3 gene (HGMW-approved symbol POUF3). Comparison of the structure of these clones with the N-Oct 3 cDNA revealed that POUF3 is an intronless gene. Sequencing of 650 bp of the promoter region showed 84% sequence identity of POUF3 with its murine homologue, the brain-2 (designated
brn-2) gene. Whereas both POUF3 and
brn-2 lack a TATA box, consensus sequences for AP-2, GCF, and SP1 transcription factors were identified within the highly conserved 5′- flanking region. These sequences may play a crucial role for the tissue-specific transcription activation of the POUF3 gene. Southern blotting and
in situ hybridization localized the human POUF3 gene to chromosome 6q16.