Lack of serological and molecular evidences of Zika virus circulation in non-human primates in three states from Brazil

• Published in: Memórias do Instituto Oswaldo Cruz Vol. 117; p. e220012
• Main Authors: Haisi, Amanda, Wu, Stacy, Zini, Nathalia, da Silva, Maria Luana Cristiny Rodrigues, Malossi, Camila Dantas, Cubas, Zalmir Silvino, Cubas, Patrícia Hoerner, Teixeira, Rodrigo Hidalgo Friciello, de Sousa, Mônica Shinneider, Lucena, Ricardo Barbosa, Svoboda, Walfrido Kühl, Osaki, Silvia Cristina, Nogueira, Mauricio Lacerda, Ullmann, Leila Sabrina, Araújo Junior, João Pessoa
• Format: Journal Article
• Language: English
• Published: Instituto Oswaldo Cruz, Ministério da Saúde 01-01-2022
• Subjects: PARASITOLOGY; TROPICAL MEDICINE; public health; non-human primates; arbovirus; sylvatic cycle
• ISSN: 0074-0276; 1678-8060; 1678-8060
• DOI: 10.1590/0074-02760220012

BACKGROUNDZika virus (ZIKV) was discovered in 1947 with the virus isolation from Rhesus monkey (Macaca mulatta) in Uganda forest, Africa. Old World Primates are involved in a sylvatic cycle of maintenance of this arbovirus, however a limited knowledge about the role of New World primates in ZIKV transmission cycles has been established. OBJECTIVEThis work aimed to investigate the presence of enzootic circulation of ZIKV in New World Primates from three Brazilian states: São Paulo, Paraíba, and Paraná. METHODSWe analyzed 100 non-human primate samples collected in 2018 and 2020 from free-ranging and captive environments from São Paulo (six municipalities belonging to Sorocaba region), Paraíba (João Pessoa municipality), and Paraná (Foz do Iguaçu municipality) using reverse transcriptase quantitative polymerase reaction (RT-qPCR) assays, indirect enzyme-linked immunosorbent assay (ELISA), and plaque reduction neutralization test (PRNT). FINDINGSAll samples (n = 141) tested negative for the presence of ZIKV genome from tissue and blood samples. In addition, all sera (n = 58) from Foz do Iguaçu' non-human primates (NHPs) were negative in serological assays. MAIN CONCLUSIONNo evidence of ZIKV circulation (molecular and serological) was found in neotropical primates. In addition, the absence of antibodies against ZIKV suggests the absence of previous viral exposure of NHPs from Foz do Iguaçu-PR.