The effects of intra-rectal and intra-peritoneal application of Origanum onites L. essential oil on 2,4,6-trinitrobenzenesulfonic acid-induced colitis in the rat

• Published in: Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie Vol. 59; no. 6; pp. 399 - 408
• Main Authors: Dundar, Emine, Olgun, Esra Gurlek, Isiksoy, Serap, Kurkcuoglu, Mine, Baser, K. Husnu Can, Bal, Cengiz
• Format: Journal Article
• Language: English
• Published: Jena Elsevier GmbH 01-04-2008; Elsevier
• Subjects: 6-Trinitrobenzenesulfonic acid-induced colitis; Administration, Rectal; Animals; Antioxidants - administration & dosage; Antioxidants - isolation & purification; Antioxidants - therapeutic use; Biological and medical sciences; Colitis - chemically induced; Colitis - drug therapy; Colitis - pathology; Colitis, Ulcerative - chemically induced; Colitis, Ulcerative - drug therapy; Colitis, Ulcerative - pathology; Colon - drug effects; Colon - metabolism; Colon - pathology; Dexamethasone - therapeutic use; Disease Models, Animal; Ethanol - toxicity; Gas Chromatography-Mass Spectrometry; Gastroenterology. Liver. Pancreas. Abdomen; Immunohistochemistry; Injections, Intraperitoneal; Intercellular Adhesion Molecule-1 - metabolism; Intestinal Mucosa - drug effects; Intestinal Mucosa - metabolism; Intestinal Mucosa - pathology; Investigative techniques, diagnostic techniques (general aspects); Medical sciences; Oils, Volatile - administration & dosage; Oils, Volatile - isolation & purification; Oils, Volatile - therapeutic use; Origanum; Origanum - chemistry; Origanum onites; Other diseases. Semiology; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Peroxidase - metabolism; Rats; Rats, Sprague-Dawley; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Treatment of inflammatory bowel disease; Trinitrobenzenesulfonic Acid - toxicity; Treatment of inflammatory bowel disease; Origanum onites; 2; Rat; Rodentia; 6-Trinitrobenzenesulfonic acid-induced colitis; Inflammatory disease; Crohn disease; Vertebrata; Anatomic pathology; Mammalia; Treatment; 4; Animal; Rectum; Digestive diseases; Intestinal disease; Essential oil; Colitis; Treatment of inflammatory bowel disease; Origanum onites; 2; Peritoneum; Ulcerative colitis
• ISSN: 0940-2993; 1618-1433
• DOI: 10.1016/j.etp.2007.11.009

The aim of the present study is to investigate the treatment efficiency of intra-rectal (IR) and intra-peritoneal (IP) application of
Origanum onites essential oil (OOEO), which is a well-known antioxidant, in the colitis model induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) and ethanol (E) in comparison with dexamethasone therapy through the morphologic damage score. Monoclonal antibodies against intercellular adhesion molecule-1 (ICAM-1, CD54), anti-rat granulocytes, and myeloperoxidase (MPO), were also investigated immunohistochemically. There was a significant difference in terms of ulceration, mucus cell depletion, inflammatory cell infiltration, vascular dilatation (
p<0.001), crypt abscesses (
p<0.01), and edema (
p<0.05) between OOEO-1
mg/kg-IR and control colitis groups. A significant difference was encountered in terms of mucus cell depletion, crypt abscesses, inflammatory cell infiltration, vascular dilatation (
p<0.01), and ulceration (
p<0.05) between the OOEO-0.1
mg/kg-IR and control colitis groups. A significant difference was noticed in terms of ulceration, inflammatory cell infiltration, mucus cell depletion (
p<0.001), vascular dilatation (
p<0.01), and mucosal atrophy (
p<0.05) between the OOEO-1
mg/kg-IP and control colitis groups. There was a significant difference in terms of ulceration, mucus cell depletion, inflammatory cell infiltration (
p<0.001), crypt abscesses, vascular dilatation (
p<0.01), and mucosal atrophy (
p<0.05) between the OOEO-0.1
mg/kg-IP and control colitis groups. No significant difference was determined in terms of ulceration, inflammatory cyst, mucosal atrophy, edema, and vascular dilatation between the dexamethazone and control colitis groups (
p>0.05). Under the present conditions, we concluded that IR and IP OOEO treatment, applied at the dosage of 0.1 or 1
mg/kg/day, have a significant protective effect on the colonic injury.