Synchrotron radiation μCT and histology evaluation of bone-to-implant contact

• Published in: Journal of cranio-maxillo-facial surgery Vol. 45; no. 9; pp. 1448 - 1457
• Main Authors: Neldam, Camilla Albeck, DDS, Ph.D. student, Sporring, Jon, Ph.D, Rack, Alexander, Dr, Lauridsen, Torsten, Hauge, Ellen-Margrethe, MD, Ph.D, Jørgensen, Henrik L., MD, Ph.D, Jørgensen, Niklas Rye, MD, DMSC, PhD, Feidenhansl, Robert, Pinholt, Else Marie, DDS, M.SCidr. odont
• Format: Journal Article
• Language: English
• Published: Scotland Elsevier Ltd 01-09-2017
• Subjects: Animals; Bone Substitutes; Bone-to-implant contact; Dental implant; Dental Implantation, Endosseous; Dental Implants; Dentistry; Female; Goats; Graft; Histology; Imaging, Three-Dimensional; Mandible - anatomy & histology; Mandible - diagnostic imaging; Osseointegration; Surgery; Synchrotron radiation micro-CT; Synchrotrons; X-Ray Microtomography; Osseointegration; Graft; Histology; Synchrotron radiation micro-CT; Dental implant; Bone-to-implant contact
• ISSN: 1010-5182; 1878-4119
• DOI: 10.1016/j.jcms.2017.05.019

The purpose of this study was to evaluate bone-to-implant contact (BIC) in two-dimensional (2D) histology compared to high-resolution three-dimensional (3D) synchrotron radiation micro computed tomography (SR micro-CT). High spatial resolution, excellent signal-to-noise ratio, and contrast establish SR micro-CT as the leading imaging modality for hard X-ray microtomography. Using SR micro-CT at voxel size 5 μm in an experimental goat mandible model, no statistically significant difference was found between the different treatment modalities nor between recipient and reconstructed bone. The histological evaluation showed a statistically significant difference between BIC in reconstructed and recipient bone (p < 0.0001). Further, no statistically significant difference was found between the different treatment modalities which we found was due to large variation and subsequently due to low power. Comparing histology and SR micro-CT evaluation a bias of 5.2% was found in reconstructed area, and 15.3% in recipient bone. We conclude that for evaluation of BIC with histology and SR micro-CT, SR micro-CT cannot be proven more precise than histology for evaluation of BIC, however, with this SR micro-CT method, one histologic bone section is comparable to the 3D evaluation. Further, the two methods complement each other with knowledge on BIC in 2D and 3D.