Molecular epidemiology of Salmonella serotype Enteritidis. Relationships between food, water and pathogenic strains

• Published in: International journal of food microbiology Vol. 43; no. 1; pp. 81 - 90
• Main Authors: Landeras, Elena, González-Hevia, M.Angeles, Mendoza, M.Carmen
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 18-08-1998; Elsevier
• Subjects: Animals; Bacteriology; Bacteriophage Typing; Biological and medical sciences; BIOLOGICAL DIFFERENCES; Cluster Analysis; DIFERENCIAS BIOLOGICAS; DIFFERENCE BIOLOGIQUE; DNA Primers - chemistry; DNA, Bacterial - chemistry; Eggs - microbiology; ENFERMEDADES TRANSM POR ALIMENTOS; Environmental; EPIDEMIOLOGIA; EPIDEMIOLOGIE; EPIDEMIOLOGY; Food industries; Food Microbiology; Food-borne; FOODBORNE DISEASES; Fundamental and applied biological sciences. Psychology; Humans; MALADIE TRANSMISSIBLE PAR ALIMENT; Meat - microbiology; Microbiology; Molecular epidemiology; Phage typing; Polymorphism, Restriction Fragment Length; Prevalence; Random Amplified Polymorphic DNA Technique; RAPD; Ribotyping; SALMONELLA ENTERITIDIS; Salmonella enteritidis - chemistry; Salmonella enteritidis - classification; Salmonella Food Poisoning - epidemiology; Salmonella serotype Enteritidis; Serotyping; Sewage - microbiology; Spain - epidemiology; Water Microbiology; Europe; Spain; Environmental; Ribotyping; RAPD; Phage typing; Molecular epidemiology; Food-borne; Salmonella serotype Enteritidis; Water; Salmonella enteritidis; Typing; Genetic marker; Ribosomal DNA; Foodstuff; Dendrogram; Virus; Random amplified polymorphic DNA; Pathogenic; Bacteria; Environment; Biological contamination; Enterobacteriaceae; Phage
• ISSN: 0168-1605; 1879-3460
• DOI: 10.1016/S0168-1605(98)00092-0

A molecular epidemiology study of
Salmonella serotype Enteritidis was carried out by ribotyping performed with a mixture of
PstI and
SphI (PS ribotyping), and randomly amplified polymorphic DNA (RAPD) typing conducted with the OPB17 primer. A series, including 38 food and 25 water strains, which were epidemiologically unrelated and collected in Spain over 1985–1996, was differentiated into 20 PS ribotypes [discrimination index (DI)=0.67], RAPD types (DI=0.28), and by combining both methods into 23 genomic groups (DI=0.76). With ribotyping data from the strains tested in this and in a previous work, including clinical and reference strains, a similarity dendrogram was traced and the subsequent branches and groupings were correlated with RAPD types, phage types and sources of origin. At a similarity level of 55%, a major cluster (grouping five subclusters and three single branches) and two minor clusters were revealed. Results supported the fact that organisms representing, at least, 40 genomic groups are currently circulating in Spain, but that only the organisms of five groups predominate and these fall into a single subcluster or lineage. Organisms of these five groups could be considered endemic, associated with food-borne human infections and, for epidemiological purposes, can be differentiated by phage typing. The most frequent phage types can be subdivided into genomic groups. Organisms of the prevalent genomic groups and several less frequent ones were mainly associated with poultry transmission and gastroenteritis as the major clinical forms, while organisms of another two frequent groups were mainly associated with extra-intestinal infections, and organisms of four infrequent groups were only collected from sewage or environmental waters.