Optimized processing of fine-needle lymph node biopsies for automated immunostaining

A straightforward, reliable technique for postcollection processing and evaluation of cytologic specimens for antigen detection using an automated immunostainer was developed. Visual assessment of cell suspension turbidity was used in parallel with light microscopic examination of concentrated cytos...

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Bibliographic Details
Published in:	Journal of veterinary diagnostic investigation Vol. 22; no. 3; pp. 383 - 388
Main Authors:	Aulbach, Adam D, Swenson, Cheryl L, Kiupel, Matti
Format:	Journal Article
Language:	English
Published:	Los Angeles, CA SAGE Publications 01-05-2010
Subjects:	accuracy animal diseases Animals automatic detection Automation B-lymphocytes biopsy Biopsy, Fine-Needle - methods Biopsy, Fine-Needle - veterinary CD3 Complex - analysis CD79 Antigens - analysis cell biology cytospin differential staining disease diagnosis dog diseases Dog Diseases - immunology Dog Diseases - pathology Dogs image analysis immunocytochemistry Immunohistochemistry - methods Immunophenotyping immunostaining lymph nodes Lymph Nodes - pathology lymphoma Lymphoma - immunology Lymphoma - pathology Lymphoma - veterinary Lymphoma, B-Cell - immunology Lymphoma, B-Cell - pathology Lymphoma, B-Cell - veterinary neoplasms T-lymphocytes validity Automated immunostaining CD79a fine-needle biopsy processing cluster of differentiation (CD)3e lymphoma dogs immunocytochemistry
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