Development of an electrode immunosensor using carbon nanofibres-gold nanoparticles-mercaptopropionic acid-polyethylenimine for chicken liver containing colistin

• Published in: Journal of agriculture and food research Vol. 15; p. 100990
• Main Authors: Kumar, Harsh, Kumar, Dinesh, Valko, Marian, Manickam, Sivakumar, Alomar, Suliman Y., Cruz-Martins, Natália, Kuča, Kamil
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-03-2024; Elsevier
• Subjects: Chicken; Colistin; Health; Immunosensor; Toxicity; Chicken; Colistin; Health; Toxicity; Immunosensor
• ISSN: 2666-1543; 2666-1543
• DOI: 10.1016/j.jafr.2024.100990

Colistin is not approved for use by humans; however, it has been used in veterinary medicine and as an additive in animal feeds for many years. This study aims to develop an amperometric immunosensor to detect colistin in chicken liver. An anti-colistin antibody was immobilised on the screen-printed electrode's surface using CNF/AuNPs/MPA/PEI to develop the immunosensor. With the help of CV measurements, this sensor was used to analyse the electrical and chemical reaction of colistin after being injected into chicken liver. Furthermore, FE-SEM and FTIR characterized the electrode surface throughout the fabrication process. Based on CV analysis, 0.072 μgkg-1 LOD was calculated with R2 value of 0.995. The results of conventional methods were compared to liver samples obtained from commercial sources to validate the immunosensor. In the constructed immunosensor, colistin had a high level of specificity, stable for more than six months despite an 11 % decline in CV current. It is imperative to highlight that the present study did not include a process of recovering colistin drug from liver tissue. This lack constitutes a constraint of our research and is annotated with suggestions for future work.
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•Colistin (polymyxin E), an antibacterial cationic peptide explicitly approved veterinary medicine and feed additive.•In the chicken liver, the maximum residue limits (MRLs) for colistin have been defined as 150μgKg-1.•The developed immunosensor has 0.72 μgKg-1 limit of detection for colistin in chicken liver.•The developed immunosensor has highly specific for colistin detection.