Cellular transcription factors that interact with p6 promoter elements of parvovirus B19

• Published in: Journal of general virology Vol. 82; no. 6; pp. 1473 - 1480
• Main Authors: Raab, Ulla, Bauer, Birgit, Gigler, Andreas, Beckenlehner, Karin, Wolf, Hans, Modrow, Susanne
• Format: Journal Article
• Language: English
• Published: England Soc General Microbiol 01-06-2001
• Subjects: Antibodies; Base Sequence; Binding, Competitive; Cell Extracts; Cell Line; Consensus Sequence - genetics; DNA - genetics; DNA - metabolism; DNA-Binding Proteins - metabolism; Erythroid-Specific DNA-Binding Factors; Gene Expression Regulation, Viral; Genes, Viral - genetics; HeLa Cells; Host Cell Factor C1; Humans; K562 Cells; Octamer Transcription Factor-1; Oligodeoxyribonucleotides - genetics; Oligodeoxyribonucleotides - metabolism; Parvovirus - genetics; Parvovirus B19; Promoter Regions, Genetic - genetics; Protein Binding; Response Elements - genetics; Sp1 Transcription Factor - metabolism; Sp3 protein; Sp3 Transcription Factor; Transcription Factors - metabolism; YY1 Transcription Factor
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-82-6-1473

Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, Franz-Josef-Strauß-Allee 11, D-93053 Regensburg, Germany 1
Author for correspondence: Susanne Modrow. Fax [phone]. e-mail susanne.modrow{at}klinik.uni-regensburg.de
All transcripts of the human parvovirus B19 identified so far are regulated by a single promoter at map unit 6 of the viral genome, the so-called p6 promoter. This promoter is active in a wide variety of different cells. In order to identify cellular transcription factors involved in regulating promoter activity, we performed gel-retardation and supershift assays using the parts of the p6 promoter sequence shown previously to be protected in footprint experiments. Thereby, binding was demonstrated of the Oct-1 protein to an octamer motif within the p6 promoter and of the transcription factor Sp1 to three GC boxes. A specific preferential interaction of the factor Sp3 with one of these boxes was observed, indicating that the ratio Sp1:Sp3 may be involved in the regulation of promoter activity. Consensus sites for the regulatory protein YY1 are located close to the GC boxes and the octamer motif, to which this factor binds efficiently.