Development of a DNA Sensor Based on Alkanethiol Self- Assembled Monolayer-Modified Electrodes

• Published in: Sensors (Basel, Switzerland) Vol. 5; no. 6; pp. 344 - 363
• Main Authors: Loaiza, Óscar A., Campuzano, Susana, López-Berlanga, María, Pedrero, María, Pingarrón, José M.
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 14-11-2005; Molecular Diversity Preservation International (MDPI)
• Subjects: Acids; Biosensors; Calf thymus DNA; denaturation; Electrodes; Gold; hybridization; methylene blue; self-assembled monolayers; Sensors; Thymus gland; Voltammetry
• ISSN: 1424-8220; 1424-8220
• DOI: 10.3390/s5060344

An electrochemical DNA biosensor based on recognition of double or singlestranded DNA (ds-DNA/ss-DNA) immobilised on a self-assembled modified gold electrodeis presented for denaturalisation and hybridisation detection. DNA is covalently bond on aself assembled 3-mercaptopropionic acid monolayer by using water soluble N-3-(dimethylaminopropyl)-N´ethylcarbodiimide hydrochloride (EDC) and Nhydroxisulfosuccinimide(NHSS) as linkers. The interaction between the immobilised DNAand methylene blue (MB) is investigated using square wave voltammetry (SWV). Theincrease or diminution of peak currents of the MB upon the hybridisation or denaturalisationevent at the modified electrode surface is studied.