Technical Aspects of Immunohistochemistry

Immunohistochemistry is an integral technique in many veterinary laboratories for diagnostic and research purposes. In the last decade, the ability to detect antigens (Ags) in tissue sections has improved dramatically, mainly by countering the deleterious effects of formaldehyde with antigen retriev...

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Bibliographic Details
Published in:	Veterinary Pathology Vol. 42; no. 4; pp. 405 - 426
Main Author:	Ramos-Vara, J. A
Format:	Journal Article Book Review
Language:	English
Published:	Los Angeles, CA SAGE Publications 01-07-2005
Subjects:	Alcohols - chemistry Antibodies - isolation & purification Antigens - isolation & purification epitopes Fixatives - chemistry Formaldehyde - chemistry immunoglobulin G Immunoglobulins - chemistry Immunohistochemistry - methods Immunohistochemistry - veterinary literature reviews Microarray Analysis - methods Microarray Analysis - veterinary Proteins - chemistry Antibodies fixation detection methods antigen retrieval immunohistochemistry antigens
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Summary:	Immunohistochemistry is an integral technique in many veterinary laboratories for diagnostic and research purposes. In the last decade, the ability to detect antigens (Ags) in tissue sections has improved dramatically, mainly by countering the deleterious effects of formaldehyde with antigen retrieval (AR) and increasing sensitivity of the detection systems. In this review, I address these topics and provide an overview of technical aspects of immunohistochemistry, including those related to antibodies (Abs) and Ags, fixation, AR, detection methods, background, and troubleshooting. Microarray technology and the use of rabbit monoclonal Abs in immunohistochemistry are also discussed.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Review-1
ISSN:	0300-9858 1544-2217
DOI:	10.1354/vp.42-4-405