Effects of salinity and temperature on inactivation and repair potential of Enterococcus faecalis following medium‐ and low‐pressure ultraviolet irradiation

• Published in: Journal of applied microbiology Vol. 120; no. 3; pp. 816 - 825
• Main Authors: Chen, P.‐Y, Chu, X.‐N, Liu, L, Hu, J.‐Y
• Format: Journal Article
• Language: English
• Published: England Published for the Society for Applied Bacteriology by Blackwell Science 01-03-2016; Oxford University Press
• Subjects: ballast water; dark repair; disinfection; Disinfection - instrumentation; Disinfection - methods; Enterococcus faecalis; Enterococcus faecalis - physiology; Enterococcus faecalis - radiation effects; fluorescent lighting; Humans; inactivation temperature; Microbiology; photoreactivation; Pressure; public health; Salinity; Temperature; Ultraviolet radiation; Ultraviolet Rays; UV disinfection; Water Purification - methods; Water treatment; Enterococcus faecalis; UV disinfection; dark repair; photoreactivation; ballast water
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/jam.13026

AIMS: To investigate the medium‐pressure (MP) and low‐pressure (LP) Ultraviolet (UV) susceptibility and the repair potential of Enterococcus faecalis (DSM 20478) after UV treatment. METHODS AND RESULTS: A range of UV doses from 4 to 19 mJ cm⁻² was selected in this study. Photoreactivation and dark repair performance were investigated under fluorescent light or in the dark respectively. The inactivation and repair performance of UV disinfection under a range of salinities (0, 1%, 3%) and temperature (4 and 25°C) were compared. Results indicated that MP UV exposure resulted in higher inactivation efficiency against Ent. faecalis than LP UV exposure. For repair potential, LP UV resulted in a greater level of light repair than MP UV. Effect of salinity on the inactivation and repair of Ent. faecalis was correlated with UV sources, whereas low temperature generally adversely affected the inactivation efficiency and final repair levels after both MP and LP UV exposure. CONCLUSIONS: Both salinity and temperature demonstrated to play an important role in the inactivation and repair capability when UV light was used to treat ballast water. SIGNIFICANCE AND IMPACT OF THE STUDY: Considering that UV‐treated ballast water is exposed or discharged to marine water environment in many countries with various temperature and salinity conditions, results of this study provide significant implications for the management of public health associated with ballast water treatment and discharge.