Highly efficient incorporation of the fluorescent nucleotide analogs tC and tCO by Klenow fragment

Studies of the mechanisms by which DNA polymerases select the correct nucleotide frequently employ fluorescently labeled DNA to monitor conformational rearrangements of the polymerase-DNA complex in response to incoming nucleotides. For this purpose, fluorescent base analogs play an increasingly imp...

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Bibliographic Details

Published in:	Nucleic acids research Vol. 37; no. 12; pp. 3924 - 3933
Main Authors:	Sandin, Peter, Stengel, Gudrun, Ljungdahl, Thomas, Börjesson, Karl, Macao, Bertil, Wilhelmsson, L. Marcus
Format:	Journal Article
Language:	English
Published:	England Oxford University Press 01-07-2009
Subjects:	Binding, Competitive Deoxyribonucleosides - chemical synthesis Deoxyribonucleosides - chemistry DNA - biosynthesis DNA - chemistry DNA Polymerase I - metabolism DNA Primers Fluorescence Polarization Fluorescent Dyes - chemistry Kinetics Molecular Biology Oxazines - chemistry Oxazines - metabolism Phenothiazines - chemistry Phenothiazines - metabolism
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