One hundred and one new microsatellite loci derived from ESTs (EST-SSRs) in bread wheat

• Published in: Theoretical and applied genetics Vol. 108; no. 7; pp. 1392 - 1400
• Main Authors: Gao, L.F, Jing, R.L, Huo, N.X, Li, Y, Li, X.P, Zhou, R.H, Chang, X.P, Tang, J.F, Ma, Z.Y, Jia, J.Z
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01-05-2004; Berlin Springer Nature B.V
• Subjects: Biological and medical sciences; Chromosome Mapping; Classical genetics, quantitative genetics, hybrids; Crosses, Genetic; Expressed Sequence Tags; Fundamental and applied biological sciences. Psychology; genetic markers; Genetics; Genetics of eukaryotes. Biological and molecular evolution; Genomics; loci; Lod Score; microsatellite repeats; Microsatellite Repeats - genetics; Phenotype; Polymorphism, Genetic; Pteridophyta, spermatophyta; Triticum; Triticum - genetics; Triticum aestivum; Vegetals; wheat; Monocotyledones; Gramineae; Microsatellite DNA; Angiospermae; Wheat bread; Genetics; Spermatophyta; Agriculture; Triticum aestivum
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-003-1554-z

Four hundred and seventy-eight microsatellite markers derived from expressed sequence tags (EST-SSRs) were screened among three mapping populations (W-7984 x Opata 85, WOpop; Lumai x Hanxuan, LHpop; Wenmai x Shanhongmai, WSpop). The number of polymorphic EST-SSR primer pairs found in WOpop, LHpop and WSpop was 92, 58 and 29 respectively. A total of 101 EST-SSR loci amplified from 88 primer sets were distributed over the 20 chromosomes of the reference maps (no markers were located on chromosome 4B). These 101 mapped EST-SSR markers add to the existing 450 microsatellite loci previously mapped in bread wheat. Seventy-four of the 101 loci showed significant similarities to known genes, including 24 genes involved in metabolism, 4 in cellular structures, 9 in stress resistance, 12 in transcription, 2 in development, 2 transporters and 21 storage proteins. Besides gliadin and glutenin, most of the 53 genes with putative functions were mapped for the first time by EST-SSR markers in bread wheat. Sequence alignment of the mapped wheat EST-SSR loci allowed tentative assignment of functionality to the other members of grasses family. Colinearity combined with homology information offers an attractive approach to comparative genomics.