RT-PCR and sequence analysis of Macrobrachium rosenbergii nodavirus: Indian isolate

• Published in: Aquaculture Vol. 252; no. 2; pp. 128 - 132
• Main Authors: Shekhar, M.S., Azad, I.S., Jithendran, K.P.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 10-03-2006; Elsevier Science; Elsevier Sequoia S.A
• Subjects: Animal aquaculture; Animal diseases; Animal productions; Aquaculture; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; General aspects; Macrobrachium rosenbergii; Macrobrachium rosenbergii nodavirus; Nodavirus; Quantitative genetics; RT-PCR; Sequence analysis; Shellfish; West Indies; India; Macrobrachium rosenbergii nodavirus; Sequence analysis; RT-PCR; Macrobrachium rosenbergii; Macrura; Crustacea; Virus; Polymerase chain reaction; Arthropoda; Nodaviridae; Decapoda; Invertebrata; Nodavirus; Aquaculture
• ISSN: 0044-8486; 1873-5622
• DOI: 10.1016/j.aquaculture.2005.07.026

White tail disease is a recently reported disease of giant freshwater prawn,
Macrobrachium rosenbergii associated with
M. rosenbergii nodavirus (
MrNV). The present study is the first report on RT-PCR and sequence analysis of
MrNV of an Indian isolate confirming the nodavirus infection in
M. rosenbergii from India. Total RNA was extracted and the cDNA was synthesized from the infected post larvae collected from a hatchery located in Chennai, India. An 850-bp amplified product could be obtained by RT-PCR. The nucleotide sequence analysis of this 850-bp segment showed 98% nucleotide and 99% amino acid sequence identity with the reported sequence of an
MrNV isolate from the West Indies.