Proteomic characterization and biological activities of the mucus produced by the zoanthid Palythoa caribaeorum (Duchassaing & Michelotti, 1860)

• Published in: Anais da Academia Brasileira de Ciências Vol. 95; no. 4; p. e20200325
• Main Authors: Silva, Marllyn M, Campos, Thiers A, Cavalcanti, Isabella M F, Oliveira, Idjane S, Pérez, Carlos Daniel, Silva, Roberto Afonso DA, Wanderley, Marcela S O, Santos, Noemia P S
• Format: Journal Article
• Language: English
• Published: Brazil Academia Brasileira de Ciências 01-01-2023
• Subjects: Animals; Anthozoa - chemistry; Anti-Bacterial Agents - pharmacology; Antibacterial; cytotoxicity; Microbial Sensitivity Tests; Mucus - microbiology; MULTIDISCIPLINARY SCIENCES; Palythoa; Proteins; proteomic; Proteomics; Palythoa; proteomic; Antibacterial; cytotoxicity
• ISSN: 0001-3765; 1678-2690; 1678-2690
• DOI: 10.1590/0001-3765202320200325

Mucus, produced by Palythoa caribaeorum has been popularly reported due to healing, anti-inflammatory, and analgesic effects. However, biochemical and pharmacological properties of this mucus remains unexplored. Therefore, the present study aimed to study its proteome profile by 2DE electrophoresis and MALDI-TOF. Furthermore, it was evaluated the cytotoxic, antibacterial, and antioxidant activities of the mucus and from its protein extract (PE). Proteomics study identified14 proteins including proteins involved in the process of tissue regeneration and death of tumor cells. The PE exhibited cell viability below 50% in the MCF-7 and S-180 strains. It showed IC50 of 6.9 μg/mL for the J774 lineage, and also, favored the cellular growth of fibroblasts. Furthermore, PE revealed activity against Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, and Staphylococcus epidermidis (MIC of 250 μg/mL). These findings revealed the mucus produced by Palythoa caribaeorum with biological activities, offering alternative therapies for the treatment of cancer and as a potential antibacterial agent.