The Internal Repeats in the Na+/Ca2+Exchanger-related Escherichia coli Protein YrbG Have Opposite Membrane Topologies

We have determined the topology of theEscherichia coli inner membrane protein YrbG, a putative Na+/Ca2+ exchanger with homology to a family of eukaryotic ion exchangers. Our results show that the two homologous halves of YrbG both have five transmembrane segments but opposite membrane orientations....

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Bibliographic Details
Published in:	The Journal of biological chemistry Vol. 276; no. 22; pp. 18905 - 18907
Main Authors:	Sääf, Annika, Baars, Louise, von Heijne, Gunnar
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01-06-2001 American Society for Biochemistry and Molecular Biology
Subjects:	Amino Acid Sequence Cell Membrane - metabolism Cytoplasm - metabolism Escherichia coli - genetics Escherichia coli - metabolism Evolution, Molecular Models, Biological Molecular Sequence Data Periplasm - metabolism Plasmids - metabolism Protein Structure, Tertiary Recombinant Fusion Proteins - metabolism Sequence Homology, Amino Acid Sodium-Calcium Exchanger - biosynthesis Sodium-Calcium Exchanger - chemistry Sodium-Calcium Exchanger - genetics
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Summary:	We have determined the topology of theEscherichia coli inner membrane protein YrbG, a putative Na+/Ca2+ exchanger with homology to a family of eukaryotic ion exchangers. Our results show that the two homologous halves of YrbG both have five transmembrane segments but opposite membrane orientations. This has implications for our understanding of the function of Na+/Ca2+ exchangers and provides an example of “divergent” evolution of membrane protein topology.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0021-9258 1083-351X
DOI:	10.1074/jbc.M101716200