Tissue Inhibitor of Metalloproteinase (TIMP)-2 Acts Synergistically with Synthetic Matrix Metalloproteinase (MMP) Inhibitors but Not with TIMP-4 to Enhance the (Membrane Type 1)-MMP-dependent Activation of Pro-MMP-2

• Published in: The Journal of biological chemistry Vol. 275; no. 52; pp. 41415 - 41423
• Main Authors: Toth, Marta, Bernardo, M. Margarida, Gervasi, David C., Soloway, Paul D., Wang, Zhiping, Bigg, Heather F., Overall, Christopher M., DeClerck, Yves A., Tschesche, Harald, Cher, Michael L., Brown, Stephen, Mobashery, Shahriar, Fridman, Rafael
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 29-12-2000; American Society for Biochemistry and Molecular Biology
• Subjects: Animals; Cell Line; Drug Synergism; Enzyme Activation; Enzyme Precursors - metabolism; Haplorhini; Humans; Matrix Metalloproteinase 2 - metabolism; Matrix Metalloproteinase Inhibitors; Matrix Metalloproteinases, Membrane-Associated; Metalloendopeptidases - physiology; Protease Inhibitors - pharmacology; Tissue Inhibitor of Metalloproteinase-2 - pharmacology; Tissue Inhibitor of Metalloproteinase-4; Tissue Inhibitor of Metalloproteinases - pharmacology
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M006871200

The membrane-type 1 matrix metalloproteinase (MT1-MMP) has been shown to be a key enzyme in tumor angiogenesis and metastasis. MT1-MMP hydrolyzes a variety of extracellular matrix components and is a physiological activator of pro-MMP-2, another MMP involved in malignancy. Pro-MMP-2 activation by MT1-MMP involves the formation of an MT1-MMP·tissue inhibitors of metalloproteinases 2 (TIMP-2)·pro-MMP-2 complex on the cell surface that promotes the hydrolysis of pro-MMP-2 by a neighboring TIMP-2-free MT1-MMP. The MT1-MMP·TIMP-2 complex also serves to reduce the intermolecular autocatalytic turnover of MT1-MMP, resulting in accumulation of active MT1-MMP (57 kDa) on the cell surface. Evidence shown here inTimp2-null cells demonstrates that pro-MMP-2 activation by MT1-MMP requires TIMP-2. In contrast, a C-terminally deleted TIMP-2 (Δ-TIMP-2), unable to form ternary complex, had no effect. However, Δ-TIMP-2 and certain synthetic MMP inhibitors, which inhibit MT1-MMP autocatalysis, can act synergistically with TIMP-2 in the promotion of pro-MMP-2 activation by MT1-MMP. In contrast, TIMP-4, an efficient MT1-MMP inhibitor, had no synergistic effect. These studies suggest that under certain conditions the pericellular activity of MT1-MMP in the presence of TIMP-2 can be modulated by synthetic and natural (TIMP-4) MMP inhibitors.