Bovine CAPN1 maps to a region of BTA29 containing a quantitative trait locus for meat tenderness

• Published in: Journal of animal science Vol. 78; no. 10; pp. 2589 - 2594
• Main Authors: Smith, T. P, Casas, E, Rexroad, C. E., 3rd, Kappes, S. M, Keele, J. W
• Format: Journal Article
• Language: English
• Published: Savoy, IL Am Soc Animal Sci 01-10-2000; American Society of Animal Science; Oxford University Press
• Subjects: Amino Acid Sequence; Animal productions; Animals; Beef; Biological and medical sciences; Calcium; calcium activated neural proteinase; Calpain - genetics; CAPN1 gene; Cattle - genetics; Chromosome Banding; Chromosome Mapping - veterinary; Deoxyribonucleic acid; DNA; Enzymes; Food industries; Fundamental and applied biological sciences. Psychology; Genes; Humans; Meat - standards; Meat and meat product industries; Molecular Sequence Data; Proteins; Quantitative Trait, Heritable; tenderness; Terrestrial animal productions; Vertebrates; Genetic mapping; Farming animal; Ruminant animal; Enzyme; Cysteine endopeptidases; Organoleptic properties; Quantitative character; Meat product; Calpain; Genetic character; Experimental study; Peptidases; Vertebrata; Meat animals; Mammalia; Tenderness; Beef cattle; Hydrolases; Artiodactyla; Beef; Ungulata
• ISSN: 0021-8812; 1525-3163; 0021-8812
• DOI: 10.2527/2000.78102589x

Micromolar calcium activated neural protease (CAPN1) was investigated as a potential candidate gene for a quantitative trait locus (QTL) on BTA29 affecting meat tenderness. A 2,948-bp bovine cDNA containing the entire coding region of the gene was obtained, showing 91% identity to human CAPN1. The 716 AA protein predicted from this sequence shows 97% similarity (95% identity) to the 714 AA human protein. Analysis of the gene structure revealed that CAPN1 mRNA is encoded by at least 19 exons, and 11,055 bp of the gene were sequenced, including 17 introns. Two single nucleotide polymorphisms (SNP) were detected in intron 12 and were used to map bovine CAPN1 to the telomeric end of the BTA29 linkage group. This approximately coincides with the position of the QTL, demonstrating that CAPN1 protease is a positional candidate gene potentially affecting variation in meat tenderness in a bovine resource mapping population.