Pituicyte Cues Regulate the Development of Permeable Neuro-Vascular Interfaces

• Published in: Developmental cell Vol. 47; no. 6; pp. 711 - 726.e5
• Main Authors: Anbalagan, Savani, Gordon, Ludmila, Blechman, Janna, Matsuoka, Ryota L., Rajamannar, Preethi, Wircer, Einav, Biran, Jakob, Reuveny, Adriana, Leshkowitz, Dena, Stainier, Didier Y.R., Levkowitz, Gil
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 17-12-2018
• Subjects: Animals; arginine-vasopressin; Astrocytes - metabolism; astroglia; blood-brain barrier; Blood-Brain Barrier - metabolism; Brain - metabolism; Claudin; Claudin-5; Cues; Endothelial Cells - metabolism; Endothelium, Vascular - metabolism; fenestrae; microglia; neuroendocrine; Neuroglia - metabolism; neurovascular unit; oxytocin; Permeability; pituicyte; Pituitary Gland - metabolism; Pituitary Gland, Posterior - cytology; Pituitary Gland, Posterior - metabolism; Pituitary Gland, Posterior - physiology; plvap; tight junction; Tight Junctions - metabolism; Up-Regulation; vascular permeability; Zebrafish; blood-brain barrier; astroglia; oxytocin; arginine-vasopressin; neuroendocrine; pituicyte; fenestrae; plvap; Claudin; zebrafish; vascular permeability; microglia; tight junction; neurovascular unit
• ISSN: 1534-5807; 1878-1551
• DOI: 10.1016/j.devcel.2018.10.017

The hypothalamo-neurohypophyseal system (HNS) regulates homeostasis through the passage of neurohormones and blood-borne proteins via permeable blood capillaries that lack the blood-brain barrier (BBB). Why neurohypophyseal capillaries become permeable while the neighboring vasculature of the brain forms BBB remains unclear. We show that pituicytes, the resident astroglial cells of the neurohypophysis, express genes that are associated with BBB breakdown during neuroinflammation. Pituicyte-enriched factors provide a local microenvironment that instructs a permeable neurovascular conduit. Thus, genetic and pharmacological perturbations of Vegfa and Tgfβ3 affected HNS vascular morphogenesis and permeability and impaired the expression of the fenestral marker plvap. The anti-inflammatory agent dexamethasone decreased HNS permeability and downregulated the pituicyte-specific cyp26b gene, encoding a retinoic acid catabolic enzyme. Inhibition of Cyp26b activity led to upregulation of tight junction protein Claudin-5 and decreased permeability. We conclude that pituicyte-derived factors regulate the “decision” of endothelial cells to adopt a permeable endothelial fate instead of forming a BBB.
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•Pituicytes, the neurohypophyseal glia, exhibit molecular similarity to astrocytes•Pituicyte factors Vegf, Tgfβ, and Cyp26b regulate vascular permeability•Vegf and Tgfβ signaling promote the permeable (i.e., fenestrated) endothelial fate•Cyp26b represses the non-permeable BBB (i.e., tight junction) endothelial fate
Why some blood vessels become permeable while majority of brain vessels form a blood-brain barrier remains unclear. Anbalagan et al. show that pituicytes, resident astroglial cells of the neurohypophysis, provide a local microenvironment that regulates the decision of endothelial cells to adopt a permeable endothelial fate instead of forming a BBB.