Analysis of BR96 Binding Sites for Antigen and Anti-Idiotype by Codon-Based Scanning Mutagenesis

• Published in: The Journal of immunology (1950) Vol. 160; no. 5; pp. 2353 - 2359
• Main Authors: Rosok, Mae Joanne, Eghtedarzadeh-Kondri, Mohammad, Young, Kelly, Bajorath, Jurgen, Glaser, Scott, Yelton, Dale
• Format: Journal Article
• Language: English
• Published: United States Am Assoc Immnol 01-03-1998
• Subjects: Animals; Antibodies, Anti-Idiotypic - biosynthesis; Antibodies, Anti-Idiotypic - chemistry; Antibodies, Anti-Idiotypic - genetics; Antibodies, Anti-Idiotypic - metabolism; Antigens - chemistry; Antigens - immunology; Antigens - metabolism; Binding Sites, Antibody - genetics; Codon - immunology; Crystallography, X-Ray; Enzyme-Linked Immunosorbent Assay; Gene Library; Immunoblotting; Immunoglobulin Heavy Chains - genetics; Immunoglobulin Heavy Chains - metabolism; Immunoglobulin Light Chains - genetics; Immunoglobulin Light Chains - metabolism; Immunoglobulin Variable Region - genetics; Immunoglobulin Variable Region - metabolism; Lewis Blood-Group System - genetics; Lewis Blood-Group System - metabolism; Mice; Mice, Inbred BALB C; Models, Molecular; Mutagenesis, Site-Directed; Recombinant Fusion Proteins - immunology; Recombinant Fusion Proteins - metabolism
• DOI: 10.4049/jimmunol.160.5.2353
• EISSN: 1550-6606
• ISSN: 0022-1767

We performed a scanning mutagenesis study of heavy chain complementarity-determining region (CDR) residues to identify how mutations affected binding of the anti-carcinoma mAb BR96 to Ag, Lewis Y, and to an anti-Id Ab (anti-Id). By ELISA, we demonstrated that the anti-Id bound close to the Ag binding site of BR96, but the anti-Id and Ag sites were not identical. Immunoblot analysis and screening of light and heavy chain CDR libraries with multiple mutations in each CDR suggested that the heavy chain had greater involvement in anti-Id binding. We then analyzed contributions of individual residues in the heavy chain CDRs to binding of Ag and anti-Id. In a filamentous phage vector containing BR96 V region sequences, mutations were introduced by codon-based mutagenesis at single positions within the three heavy chain CDRs. The resulting libraries of Fab fragments had all amino acids represented at a CDR position. We evaluated the expressed Fabs for binding to Ag and anti-Id by plaque lift assay. We identified the positions with mutations that had the greatest negative effect on binding to the anti-Id and to Ag and analyzed them on the basis of the BR96 x-ray structure. The residues most important for binding to the anti-Id were located in heavy chain CDR1 and CDR2 and were peripheral to the residues within the Lewis Y binding pocket.