A novel expression cloning method to isolate mammalian transcription factors in Schizosaccharomyces pombe

We describe a novel expression cloning strategy in the fission yeast for the isolation of mammalian transcription factors using a mammalian promoter as target. This strategy is possible because of the conservation between mammalian cells and Schizosaccharomyces pombe of the mechanism that leads to t...

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Bibliographic Details

Published in:	Nucleic acids research Vol. 26; no. 22; pp. 5223 - 5224
Main Authors:	Remacle, Jacques E., Brys, Reginald, Pype, Stefan, Nelles, Luc, Huylebroeck, Danny
Format:	Journal Article
Language:	English
Published:	England Oxford University Press 15-11-1998
Subjects:	Cells, Cultured Cloning, Molecular - methods DNA, Complementary - genetics Gene Expression Humans Promoter Regions, Genetic Schizosaccharomyces - genetics Schizosaccharomyces pombe Transcription Factors - genetics Transcription Factors - isolation & purification Transcription, Genetic Transfection Vascular Cell Adhesion Molecule-1 - genetics
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