UV and chemical mutagenesis in rev7 mutants of yeast

• Published in: Molecular & general genetics Vol. 200; no. 1; p. 86
• Main Authors: Lawrence, C W, Nisson, P E, Christensen, R B
• Format: Journal Article
• Language: English
• Published: Germany 01-01-1985
• Subjects: Alleles; Diploidy; Ethyl Methanesulfonate - pharmacology; Genes, Fungal - drug effects; Genes, Fungal - radiation effects; Methyl Methanesulfonate - pharmacology; Methylnitronitrosoguanidine - pharmacology; Mutagens - pharmacology; Mutation; Saccharomyces cerevisiae - drug effects; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - radiation effects; Ultraviolet Rays
• ISSN: 0026-8925
• DOI: 10.1007/bf00383317

We have examined induced mutagenesis in rev7-1 mutants of Baker's yeast' Saccharomyces cerevisiae, using a variety of contrasting test systems and several different mutagens. UV-induced reversion frequencies of the ochre allele arg4-17, the putative missense allele ilv1-92 and the frameshift allele his4-38 were 10 to 200 fold lower in haploid and diploid rev7-1 mutants compared with wild type strains, but UV-induced reversion frequencies of the frameshift allele leu2-3 and the proline missense allele cyc1-115 were reduced only a few fold. Ilv1-92 reversion frequencies induced by methyl methane sulfonate or by N-methyl-N'-nitro-N-nitrosoguanidine were 10 to 20 times lower in rev7-1 mutants, but normal frequencies of these revertants were induced with ethyl methane sulfonate, even though rev7-1 strains are slightly sensitive to this mutagen as well as to the others tested. We conclude that the rev7 mutants, like the rev3 mutants they closely resemble, have a substantial but not total deficiency concerning induced mutagenesis.