Investigation of cardiac fibroblasts using myocardial slices

Abstract Aims Cardiac fibroblasts (CFs) are considered the principal regulators of cardiac fibrosis. Factors that influence CF activity are difficult to determine. When isolated and cultured in vitro, CFs undergo rapid phenotypic changes including increased expression of α-SMA. Here we describe a ne...

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Published in:	Cardiovascular research Vol. 114; no. 1; pp. 77 - 89
Main Authors:	Perbellini, Filippo, Watson, Samuel A, Scigliano, Martina, Alayoubi, Samha, Tkach, Sebastian, Bardi, Ifigeneia, Quaife, Nicholas, Kane, Christopher, Dufton, Neil P, Simon, André, Sikkel, Markus B, Faggian, Giuseppe, Randi, Anna M, Gorelik, Julia, Harding, Sian E, Terracciano, Cesare M
Format:	Journal Article
Language:	English
Published:	England Oxford University Press 01-01-2018
Subjects:	Actins - metabolism Angiotensin II - metabolism Animals Biomarkers - metabolism Cell Proliferation - drug effects Collagen - metabolism Dogs Fibroblasts - drug effects Fibroblasts - metabolism Fibroblasts - pathology Fibrosis Humans Mice, Transgenic Myocardium - metabolism Myocardium - pathology Original Phenotype Physical Stimulation Time Factors Tissue Culture Techniques Transforming Growth Factor beta - pharmacology Vimentin - metabolism Mechanical load α-SMA Cardiac fibroblasts Myocardial slices Fibrosis
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Abstract	Abstract Aims Cardiac fibroblasts (CFs) are considered the principal regulators of cardiac fibrosis. Factors that influence CF activity are difficult to determine. When isolated and cultured in vitro, CFs undergo rapid phenotypic changes including increased expression of α-SMA. Here we describe a new model to study CFs and their response to pharmacological and mechanical stimuli using in vitro cultured mouse, dog and human myocardial slices. Methods and results Unloading of myocardial slices induced CF proliferation without α-SMA expression up to 7 days in culture. CFs migrating onto the culture plastic support or cultured on glass expressed αSMA within 3 days. The cells on the slice remained αSMA(−) despite transforming growth factor-β (20 ng/ml) or angiotensin II (200 µM) stimulation. When diastolic load was applied to myocardial slices using A-shaped stretchers, CF proliferation was significantly prevented at Days 3 and 7 (P < 0.001). Conclusions Myocardial slices allow the study of CFs in a multicellular environment and may be used to effectively study mechanisms of cardiac fibrosis and potential targets.
AbstractList	Abstract Aims Cardiac fibroblasts (CFs) are considered the principal regulators of cardiac fibrosis. Factors that influence CF activity are difficult to determine. When isolated and cultured in vitro, CFs undergo rapid phenotypic changes including increased expression of α-SMA. Here we describe a new model to study CFs and their response to pharmacological and mechanical stimuli using in vitro cultured mouse, dog and human myocardial slices. Methods and results Unloading of myocardial slices induced CF proliferation without α-SMA expression up to 7 days in culture. CFs migrating onto the culture plastic support or cultured on glass expressed αSMA within 3 days. The cells on the slice remained αSMA(−) despite transforming growth factor-β (20 ng/ml) or angiotensin II (200 µM) stimulation. When diastolic load was applied to myocardial slices using A-shaped stretchers, CF proliferation was significantly prevented at Days 3 and 7 (P < 0.001). Conclusions Myocardial slices allow the study of CFs in a multicellular environment and may be used to effectively study mechanisms of cardiac fibrosis and potential targets. Cardiac fibroblasts (CFs) are considered the principal regulators of cardiac fibrosis. Factors that influence CF activity are difficult to determine. When isolated and cultured in vitro, CFs undergo rapid phenotypic changes including increased expression of α-SMA. Here we describe a new model to study CFs and their response to pharmacological and mechanical stimuli using in vitro cultured mouse, dog and human myocardial slices. Unloading of myocardial slices induced CF proliferation without α-SMA expression up to 7 days in culture. CFs migrating onto the culture plastic support or cultured on glass expressed αSMA within 3 days. The cells on the slice remained αSMA(-) despite transforming growth factor-β (20 ng/ml) or angiotensin II (200 µM) stimulation. When diastolic load was applied to myocardial slices using A-shaped stretchers, CF proliferation was significantly prevented at Days 3 and 7 (P < 0.001). Myocardial slices allow the study of CFs in a multicellular environment and may be used to effectively study mechanisms of cardiac fibrosis and potential targets. AimsCardiac fibroblasts (CFs) are considered the principal regulators of cardiac fibrosis. Factors that influence CF activity are difficult to determine. When isolated and cultured in vitro, CFs undergo rapid phenotypic changes including increased expression of α-SMA. Here we describe a new model to study CFs and their response to pharmacological and mechanical stimuli using in vitro cultured mouse, dog and human myocardial slices. Methods and resultsUnloading of myocardial slices induced CF proliferation without α-SMA expression up to 7 days in culture. CFs migrating onto the culture plastic support or cultured on glass expressed αSMA within 3 days. The cells on the slice remained αSMA(-) despite transforming growth factor-β (20 ng/ml) or angiotensin II (200 µM) stimulation. When diastolic load was applied to myocardial slices using A-shaped stretchers, CF proliferation was significantly prevented at Days 3 and 7 (P < 0.001). ConclusionsMyocardial slices allow the study of CFs in a multicellular environment and may be used to effectively study mechanisms of cardiac fibrosis and potential targets.
Author	Quaife, Nicholas Scigliano, Martina Sikkel, Markus B Faggian, Giuseppe Harding, Sian E Perbellini, Filippo Tkach, Sebastian Simon, André Alayoubi, Samha Randi, Anna M Watson, Samuel A Dufton, Neil P Kane, Christopher Bardi, Ifigeneia Gorelik, Julia Terracciano, Cesare M
AuthorAffiliation	2 Department of Cardiac Surgery, University of Verona , Verona, Italy 1 Imperial Centre for Translational and Experimental Medicine, National Heart and Lung Institute, Imperial College London, Hammersmith Campus , Du Cane Road, London, UK 3 Department of Cardiothoracic Transplantation and Mechanical Circulatory Support, Royal Brompton and Harefield NHS Foundation Trust , Harefield, UK
AuthorAffiliation_xml	– name: 2 Department of Cardiac Surgery, University of Verona , Verona, Italy – name: 3 Department of Cardiothoracic Transplantation and Mechanical Circulatory Support, Royal Brompton and Harefield NHS Foundation Trust , Harefield, UK – name: 1 Imperial Centre for Translational and Experimental Medicine, National Heart and Lung Institute, Imperial College London, Hammersmith Campus , Du Cane Road, London, UK
Author_xml	– sequence: 1 givenname: Filippo surname: Perbellini fullname: Perbellini, Filippo organization: Imperial Centre for Translational and Experimental Medicine, National Heart and Lung Institute, Imperial College London, Hammersmith Campus, Du Cane Road, London W12 0NN, UK – sequence: 2 givenname: Samuel A surname: Watson fullname: Watson, Samuel A organization: Imperial Centre for Translational and Experimental Medicine, National Heart and Lung Institute, Imperial College London, Hammersmith Campus, Du Cane Road, London W12 0NN, UK – sequence: 3 givenname: Martina surname: Scigliano fullname: Scigliano, Martina organization: Department of Cardiac Surgery, University of Verona, Verona, Italy – sequence: 4 givenname: Samha surname: Alayoubi fullname: Alayoubi, Samha organization: Imperial Centre for Translational and Experimental Medicine, National Heart and Lung Institute, Imperial College London, Hammersmith Campus, Du Cane Road, London W12 0NN, UK – sequence: 5 givenname: Sebastian surname: Tkach fullname: Tkach, Sebastian organization: Imperial Centre for Translational and Experimental Medicine, National Heart and Lung Institute, Imperial College London, Hammersmith Campus, Du Cane Road, London W12 0NN, UK – sequence: 6 givenname: Ifigeneia surname: Bardi fullname: Bardi, Ifigeneia organization: Imperial Centre for Translational and Experimental Medicine, National Heart and Lung Institute, Imperial College London, Hammersmith Campus, Du Cane Road, London W12 0NN, UK – sequence: 7 givenname: Nicholas surname: Quaife fullname: Quaife, Nicholas organization: Imperial Centre for Translational and Experimental Medicine, National Heart and Lung Institute, Imperial College London, Hammersmith Campus, Du Cane Road, London W12 0NN, UK – sequence: 8 givenname: Christopher surname: Kane fullname: Kane, Christopher organization: Imperial Centre for Translational and Experimental Medicine, National Heart and Lung Institute, Imperial College London, Hammersmith Campus, Du Cane Road, London W12 0NN, UK – sequence: 9 givenname: Neil P surname: Dufton fullname: Dufton, Neil P organization: Imperial Centre for Translational and Experimental Medicine, National Heart and Lung Institute, Imperial College London, Hammersmith Campus, Du Cane Road, London W12 0NN, UK – sequence: 10 givenname: André surname: Simon fullname: Simon, André organization: Department of Cardiothoracic Transplantation and Mechanical Circulatory Support, Royal Brompton and Harefield NHS Foundation Trust, Harefield, UK – sequence: 11 givenname: Markus B surname: Sikkel fullname: Sikkel, Markus B organization: Imperial Centre for Translational and Experimental Medicine, National Heart and Lung Institute, Imperial College London, Hammersmith Campus, Du Cane Road, London W12 0NN, UK – sequence: 12 givenname: Giuseppe surname: Faggian fullname: Faggian, Giuseppe organization: Department of Cardiac Surgery, University of Verona, Verona, Italy – sequence: 13 givenname: Anna M surname: Randi fullname: Randi, Anna M organization: Imperial Centre for Translational and Experimental Medicine, National Heart and Lung Institute, Imperial College London, Hammersmith Campus, Du Cane Road, London W12 0NN, UK – sequence: 14 givenname: Julia surname: Gorelik fullname: Gorelik, Julia organization: Imperial Centre for Translational and Experimental Medicine, National Heart and Lung Institute, Imperial College London, Hammersmith Campus, Du Cane Road, London W12 0NN, UK – sequence: 15 givenname: Sian E surname: Harding fullname: Harding, Sian E organization: Imperial Centre for Translational and Experimental Medicine, National Heart and Lung Institute, Imperial College London, Hammersmith Campus, Du Cane Road, London W12 0NN, UK – sequence: 16 givenname: Cesare M surname: Terracciano fullname: Terracciano, Cesare M email: c.terracciano@imperial.ac.uk organization: Imperial Centre for Translational and Experimental Medicine, National Heart and Lung Institute, Imperial College London, Hammersmith Campus, Du Cane Road, London W12 0NN, UK
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Cites_doi	10.1371/journal.pone.0052032 10.1016/j.yjmcc.2015.02.025 10.1152/ajpheart.00556.2014 10.1016/j.carpath.2010.10.001 10.1371/journal.pone.0115991 10.1093/cvr/cvt338 10.1016/j.actbio.2012.01.006 10.1016/j.vascn.2011.12.002 10.1016/j.yjmcc.2008.12.016 10.1161/01.CIR.88.6.2849 10.1161/CIRCULATIONAHA.110.982512 10.1093/cvr/cvn299 10.1146/annurev.bi.57.070188.003113 10.1016/S0002-9440(10)65334-5 10.1038/nrcardio.2009.199 10.1186/1755-1536-5-15 10.1161/CIRCRESAHA.116.308139 10.1529/biophysj.104.047308 10.1016/j.devcel.2014.11.016 10.1016/j.yjmcc.2013.12.024 10.1038/srep28798 10.1073/pnas.0608799103 10.1093/cvr/cvx151 10.1002/dvg.20367 10.1016/j.yjmcc.2014.02.017 10.1161/CIRCRESAHA.115.307778 10.3317/jraas.2000.064 10.1097/FJC.0b013e3182137e17 10.1016/j.yjmcc.2013.12.020 10.1016/j.yjmcc.2015.08.016 10.1038/nm1613 10.1093/cvr/cvr259 10.1016/j.jacc.2010.04.019 10.1113/expphysiol.2007.040659 10.1111/j.1365-2362.2011.02549.x 10.1161/CIRCRESAHA.109.209809 10.1161/CIRCRESAHA.110.231910 10.1016/S0008-6363(00)00030-4 10.1161/CIRCRESAHA.116.309202 10.1038/ncomms12260 10.1016/j.yjmcc.2011.06.018 10.1016/j.pharmthera.2009.05.002 10.1016/j.cardiores.2004.08.020 10.1161/CIRCRESAHA.116.305381 10.1161/CIRCULATIONAHA.104.515106 10.1006/jmcc.1996.0185 10.1016/j.yjmcc.2013.11.015 10.1038/nrm809
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Keywords	Mechanical load α-SMA Cardiac fibroblasts Myocardial slices Fibrosis
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References	Haudek (2019091808530319400_cvx152-B46) 2006; 103 Shyu (2019091808530319400_cvx152-B22) 2012; 42 Sigel (2019091808530319400_cvx152-B34) 1996; 28 Bussek (2019091808530319400_cvx152-B26) 2012; 66 Banyasz (2019091808530319400_cvx152-B43) 2008; 93 Stewart (2019091808530319400_cvx152-B51) 2011; 123 Pinto (2019091808530319400_cvx152-B7) 2016; 118 Leask (2019091808530319400_cvx152-B15) 2015; 116 Villarreal (2019091808530319400_cvx152-B31) 1993; 88 Benamer (2019091808530319400_cvx152-B19) 2009; 46 Galie (2019091808530319400_cvx152-B21) 2011; 20 Bei (2019091808530319400_cvx152-B9) 2015; 10 Fan (2019091808530319400_cvx152-B39) 2012; 5 Ackers-Johnson (2019091808530319400_cvx152-B53) 2016; 119 Upadhya (2019091808530319400_cvx152-B2) 2015; 83 Borne (2019091808530319400_cvx152-B11) 2010; 7 Zeisberg (2019091808530319400_cvx152-B14) 2010; 107 Arora (2019091808530319400_cvx152-B20) 1999; 154 Gould (2019091808530319400_cvx152-B41) 2012; 8 Kohl (2019091808530319400_cvx152-B50) 2014; 70 Steinert (2019091808530319400_cvx152-B10) 1988; 57 Habeler (2019091808530319400_cvx152-B28) 2009; 81 Diaz-Flores (2019091808530319400_cvx152-B47) 2009; 24 Kang (2019091808530319400_cvx152-B25) 2016; 6 Porter (2019091808530319400_cvx152-B13) 2009; 123 Shinde (2019091808530319400_cvx152-B5) 2014; 70 Williams (2019091808530319400_cvx152-B32) 2005; 88 MacKenna (2019091808530319400_cvx152-B42) 2000; 46 Tomasek (2019091808530319400_cvx152-B48) 2002; 3 Dawson (2019091808530319400_cvx152-B18) 2012; 7 Drakos (2019091808530319400_cvx152-B35) 2010; 56 Souders (2019091808530319400_cvx152-B6) 2009; 105 Petrov (2019091808530319400_cvx152-B38) 2000; 1 Brandenburger (2019091808530319400_cvx152-B30) 2012; 93 Claxton (2019091808530319400_cvx152-B29) 2008; 46 Deb (2019091808530319400_cvx152-B8) 2014; 70 Wang (2019091808530319400_cvx152-B24) 2015; 308 Driesen (2019091808530319400_cvx152-B36) 2014; 101 Camelliti (2019091808530319400_cvx152-B12) 2005; 65 Rohr (2019091808530319400_cvx152-B17) 2011; 57 Birdsey (2019091808530319400_cvx152-B37) 2015; 32 Cartledge (2019091808530319400_cvx152-B4) 2013 Strieter (2019091808530319400_cvx152-B45) 2009; 120 Tillmanns (2019091808530319400_cvx152-B49) 2015; 87 Camelliti (2019091808530319400_cvx152-B23) 2011; 51 Sikkel (2019091808530319400_cvx152-B33) 2017; 113 Zeisberg (2019091808530319400_cvx152-B44) 2007; 13 Biernacka (2019091808530319400_cvx152-B1) 2011; 2 Klotz (2019091808530319400_cvx152-B40) 2005; 112 Abramochkin (2019091808530319400_cvx152-B3) 2014; 70 Kanisicak (2019091808530319400_cvx152-B16) 2016; 7 Kang (2019091808530319400_cvx152-B27) 2016; 6 Zhou (2019091808530319400_cvx152-B52) 2016; 118
References_xml	– volume: 7 start-page: e52032. year: 2012 ident: 2019091808530319400_cvx152-B18 article-title: Congestive heart failure effects on atrial fibroblast phenotype: differences between freshly-isolated and cultured cells publication-title: PLoS One doi: 10.1371/journal.pone.0052032 contributor: fullname: Dawson – volume: 83 start-page: 73 year: 2015 ident: 2019091808530319400_cvx152-B2 article-title: Heart failure with preserved ejection fraction in the elderly: scope of the problem publication-title: J Mol Cell Cardiol doi: 10.1016/j.yjmcc.2015.02.025 contributor: fullname: Upadhya – volume: 308 start-page: H1112 year: 2015 ident: 2019091808530319400_cvx152-B24 article-title: Cardiac tissue slices: preparation, handling, and successful optical mapping publication-title: Am J Physiol Heart Circ Physiol doi: 10.1152/ajpheart.00556.2014 contributor: fullname: Wang – volume: 20 start-page: 325 year: 2011 ident: 2019091808530319400_cvx152-B21 article-title: Reduced serum content and increased matrix stiffness promote the cardiac myofibroblast transition in 3D collagen matrices publication-title: Cardiovasc Pathol doi: 10.1016/j.carpath.2010.10.001 contributor: fullname: Galie – volume: 10 start-page: e0115991. year: 2015 ident: 2019091808530319400_cvx152-B9 article-title: Cardiac telocytes and fibroblasts in primary culture: different morphologies and immunophenotypes publication-title: PLoS One doi: 10.1371/journal.pone.0115991 contributor: fullname: Bei – volume: 101 start-page: 411 year: 2014 ident: 2019091808530319400_cvx152-B36 article-title: Reversible and irreversible differentiation of cardiac fibroblasts publication-title: Cardiovasc Res doi: 10.1093/cvr/cvt338 contributor: fullname: Driesen – volume: 8 start-page: 1710 year: 2012 ident: 2019091808530319400_cvx152-B41 article-title: Cyclic strain anisotropy regulates valvular interstitial cell phenotype and tissue remodeling in three-dimensional culture publication-title: Acta Biomater doi: 10.1016/j.actbio.2012.01.006 contributor: fullname: Gould – volume: 2 start-page: 158 year: 2011 ident: 2019091808530319400_cvx152-B1 article-title: Aging and Cardiac Fibrosis publication-title: Aging Dis contributor: fullname: Biernacka – volume: 6 start-page: 1 year: 2016 ident: 2019091808530319400_cvx152-B25 article-title: Human organotypic cultured cardiac slices: new platform for high throughput preclinical human trials publication-title: Sci Rep contributor: fullname: Kang – volume: 66 start-page: 145 year: 2012 ident: 2019091808530319400_cvx152-B26 article-title: Cardiac tissue slices with prolonged survival for in vitro drug safety screening publication-title: J Pharmacol Toxicol Methods doi: 10.1016/j.vascn.2011.12.002 contributor: fullname: Bussek – volume: 46 start-page: 508 year: 2009 ident: 2019091808530319400_cvx152-B19 article-title: Molecular and functional characterization of a new potassium conductance in mouse ventricular fibroblasts publication-title: J Mol Cell Cardiol doi: 10.1016/j.yjmcc.2008.12.016 contributor: fullname: Benamer – volume: 88 start-page: 2849 year: 1993 ident: 2019091808530319400_cvx152-B31 article-title: Identification of functional angiotensin II receptors on rat cardiac fibroblasts publication-title: Circulation doi: 10.1161/01.CIR.88.6.2849 contributor: fullname: Villarreal – volume: 123 start-page: 1559 year: 2011 ident: 2019091808530319400_cvx152-B51 article-title: Keeping left ventricular assist device acceleration on track publication-title: Circulation doi: 10.1161/CIRCULATIONAHA.110.982512 contributor: fullname: Stewart – volume: 81 start-page: 253 year: 2009 ident: 2019091808530319400_cvx152-B28 article-title: An in vitro beating heart model for long-term assessment of experimental therapeutics publication-title: Cardiovasc Res doi: 10.1093/cvr/cvn299 contributor: fullname: Habeler – volume: 57 start-page: 593 year: 1988 ident: 2019091808530319400_cvx152-B10 article-title: Molecular and cellular biology of intermediate filaments publication-title: Annu Rev Biochem doi: 10.1146/annurev.bi.57.070188.003113 contributor: fullname: Steinert – volume: 154 start-page: 871 year: 1999 ident: 2019091808530319400_cvx152-B20 article-title: The compliance of collagen gels regulates transforming growth factor-beta induction of alpha-smooth muscle actin in fibroblasts publication-title: Am J Pathol doi: 10.1016/S0002-9440(10)65334-5 contributor: fullname: Arora – volume: 7 start-page: 30 year: 2010 ident: 2019091808530319400_cvx152-B11 article-title: Myocardial remodeling after infarction: the role of myofibroblasts publication-title: Nat Rev Cardiol doi: 10.1038/nrcardio.2009.199 contributor: fullname: Borne – volume: 5 start-page: 15. year: 2012 ident: 2019091808530319400_cvx152-B39 article-title: Cardiac fibroblasts, fibrosis and extracellular matrix remodeling in heart disease publication-title: Fibrogenesis Tissue Repair doi: 10.1186/1755-1536-5-15 contributor: fullname: Fan – volume: 118 start-page: 368 year: 2016 ident: 2019091808530319400_cvx152-B52 article-title: Recounting cardiac cellular composition publication-title: Circ Res doi: 10.1161/CIRCRESAHA.116.308139 contributor: fullname: Zhou – volume: 88 start-page: 1377 year: 2005 ident: 2019091808530319400_cvx152-B32 article-title: Interpreting second-harmonic generation images of collagen I fibrils publication-title: Biophys J doi: 10.1529/biophysj.104.047308 contributor: fullname: Williams – volume: 32 start-page: 82 year: 2015 ident: 2019091808530319400_cvx152-B37 article-title: The endothelial transcription factor ERG promotes vascular stability and growth through Wnt/β-catenin signaling publication-title: Dev Cell doi: 10.1016/j.devcel.2014.11.016 contributor: fullname: Birdsey – volume: 70 start-page: 37 year: 2014 ident: 2019091808530319400_cvx152-B50 article-title: Fibroblast-myocyte electrotonic coupling: does it occur in native cardiac tissue? publication-title: J Mol Cell Cardiol doi: 10.1016/j.yjmcc.2013.12.024 contributor: fullname: Kohl – volume: 6 start-page: 28798. year: 2016 ident: 2019091808530319400_cvx152-B27 article-title: Human organotypic cultured cardiac slices: new platform for high throughput preclinical human trials publication-title: Sci Rep doi: 10.1038/srep28798 contributor: fullname: Kang – volume: 103 start-page: 18284 year: 2006 ident: 2019091808530319400_cvx152-B46 article-title: Bone marrow-derived fibroblast precursors mediate ischemic cardiomyopathy in mice publication-title: Proc Natl Acad Sci USA doi: 10.1073/pnas.0608799103 contributor: fullname: Haudek – volume: 113 start-page: 1743 year: 2017 ident: 2019091808530319400_cvx152-B33 article-title: Hierarchical statistical techniques are necessary to draw reliable conclusions from analysis of isolated cardiomyocyte studies publication-title: Cardiovasc Res doi: 10.1093/cvr/cvx151 contributor: fullname: Sikkel – volume: 46 start-page: 74 year: 2008 ident: 2019091808530319400_cvx152-B29 article-title: Efficient, inducible Cre-recombinase activation in vascular endothelium publication-title: Genesis doi: 10.1002/dvg.20367 contributor: fullname: Claxton – volume: 70 start-page: 47 year: 2014 ident: 2019091808530319400_cvx152-B8 article-title: Cardiac fibroblast in development and wound healing publication-title: J Mol Cell Cardiol doi: 10.1016/j.yjmcc.2014.02.017 contributor: fullname: Deb – volume: 118 start-page: 400 year: 2016 ident: 2019091808530319400_cvx152-B7 article-title: Revisiting Cardiac Cellular CompositionNovelty and Significance publication-title: Circ Res doi: 10.1161/CIRCRESAHA.115.307778 contributor: fullname: Pinto – year: 2013 ident: 2019091808530319400_cvx152-B4 contributor: fullname: Cartledge – volume: 1 start-page: 342 year: 2000 ident: 2019091808530319400_cvx152-B38 article-title: Transforming growth factor-beta(1) induces angiotensin-converting enzyme synthesis in rat cardiac fibroblasts during their differentiation to myofibroblasts publication-title: J Renin Angiotensin Aldosterone Syst doi: 10.3317/jraas.2000.064 contributor: fullname: Petrov – volume: 57 start-page: 389 year: 2011 ident: 2019091808530319400_cvx152-B17 article-title: Cardiac fibroblasts in cell culture systems: myofibroblasts all along? publication-title: J Cardiovasc Pharmacol doi: 10.1097/FJC.0b013e3182137e17 contributor: fullname: Rohr – volume: 70 start-page: 27 year: 2014 ident: 2019091808530319400_cvx152-B3 article-title: Influence of mechanical stress on fibroblast-myocyte interactions in mammalian heart publication-title: J Mol Cell Cardiol doi: 10.1016/j.yjmcc.2013.12.020 contributor: fullname: Abramochkin – volume: 87 start-page: 194 year: 2015 ident: 2019091808530319400_cvx152-B49 article-title: Fibroblast activation protein alpha expression identifies activated fibroblasts after myocardial infarction publication-title: J Mol Cell Cardiol doi: 10.1016/j.yjmcc.2015.08.016 contributor: fullname: Tillmanns – volume: 13 start-page: 952 year: 2007 ident: 2019091808530319400_cvx152-B44 article-title: Endothelial-to-mesenchymal transition contributes to cardiac fibrosis publication-title: Nat Med doi: 10.1038/nm1613 contributor: fullname: Zeisberg – volume: 93 start-page: 50 year: 2012 ident: 2019091808530319400_cvx152-B30 article-title: Organotypic slice culture from human adult ventricular myocardium publication-title: Cardiovasc Res doi: 10.1093/cvr/cvr259 contributor: fullname: Brandenburger – volume: 56 start-page: 382 year: 2010 ident: 2019091808530319400_cvx152-B35 article-title: Impact of mechanical unloading on microvasculature and associated central remodeling features of the failing human heart publication-title: J Am Coll Cardiol C doi: 10.1016/j.jacc.2010.04.019 contributor: fullname: Drakos – volume: 93 start-page: 370 year: 2008 ident: 2019091808530319400_cvx152-B43 article-title: Transformation of adult rat cardiac myocytes in primary culture publication-title: Exp Physiol doi: 10.1113/expphysiol.2007.040659 contributor: fullname: Banyasz – volume: 42 start-page: 17 year: 2012 ident: 2019091808530319400_cvx152-B22 article-title: Angiotensin II mediates urotensin II expression by hypoxia in cultured cardiac fibroblast publication-title: Eur J Clin Invest doi: 10.1111/j.1365-2362.2011.02549.x contributor: fullname: Shyu – volume: 105 start-page: 1164 year: 2009 ident: 2019091808530319400_cvx152-B6 article-title: Cardiac fibroblast: the renaissance cell publication-title: Circ Res doi: 10.1161/CIRCRESAHA.109.209809 contributor: fullname: Souders – volume: 107 start-page: 1304 year: 2010 ident: 2019091808530319400_cvx152-B14 article-title: Origins of cardiac fibroblasts publication-title: Circ Res doi: 10.1161/CIRCRESAHA.110.231910 contributor: fullname: Zeisberg – volume: 46 start-page: 257 year: 2000 ident: 2019091808530319400_cvx152-B42 article-title: Role of mechanical factors in modulating cardiac fibroblast function and extracellular matrix synthesis publication-title: Cardiovasc Res doi: 10.1016/S0008-6363(00)00030-4 contributor: fullname: MacKenna – volume: 119 start-page: 909 year: 2016 ident: 2019091808530319400_cvx152-B53 article-title: A simplified, langendorff-free method for concomitant isolation of viable cardiac myocytes and nonmyocytes from the adult mouse heart publication-title: Circ Res doi: 10.1161/CIRCRESAHA.116.309202 contributor: fullname: Ackers-Johnson – volume: 7 start-page: 12260. year: 2016 ident: 2019091808530319400_cvx152-B16 article-title: Genetic lineage tracing defines myofibroblast origin and function in the injured heart publication-title: Nat Comms doi: 10.1038/ncomms12260 contributor: fullname: Kanisicak – volume: 51 start-page: 390 year: 2011 ident: 2019091808530319400_cvx152-B23 article-title: Adult human heart slices are a multicellular system suitable for electrophysiological and pharmacological studies publication-title: J Mol Cell Cardiol doi: 10.1016/j.yjmcc.2011.06.018 contributor: fullname: Camelliti – volume: 123 start-page: 255 year: 2009 ident: 2019091808530319400_cvx152-B13 article-title: Cardiac fibroblasts: at the heart of myocardial remodeling publication-title: Pharmacol Ther doi: 10.1016/j.pharmthera.2009.05.002 contributor: fullname: Porter – volume: 65 start-page: 40 year: 2005 ident: 2019091808530319400_cvx152-B12 article-title: Structural and functional characterisation of cardiac fibroblasts publication-title: Cardiovasc Res doi: 10.1016/j.cardiores.2004.08.020 contributor: fullname: Camelliti – volume: 116 start-page: 1269 year: 2015 ident: 2019091808530319400_cvx152-B15 article-title: Getting to the heart of the matter: new insights into cardiac fibrosis publication-title: Circ Res doi: 10.1161/CIRCRESAHA.116.305381 contributor: fullname: Leask – volume: 24 start-page: 909 year: 2009 ident: 2019091808530319400_cvx152-B47 article-title: Pericytes. Morphofunction, interactions and pathology in a quiescent and activated mesenchymal cell niche publication-title: Histol Histopathol contributor: fullname: Diaz-Flores – volume: 112 start-page: 364 year: 2005 ident: 2019091808530319400_cvx152-B40 article-title: Mechanical unloading during left ventricular assist device support increases left ventricular collagen cross-linking and myocardial stiffness publication-title: Circulation doi: 10.1161/CIRCULATIONAHA.104.515106 contributor: fullname: Klotz – volume: 28 start-page: 1921 year: 1996 ident: 2019091808530319400_cvx152-B34 article-title: Regulation of proliferative response of cardiac fibroblasts by transforming growth factor-β1 publication-title: J Mol Cell Cardiol doi: 10.1006/jmcc.1996.0185 contributor: fullname: Sigel – volume: 70 start-page: 74 year: 2014 ident: 2019091808530319400_cvx152-B5 article-title: Fibroblasts in myocardial infarction: a role in inflammation and repair publication-title: J Mol Cell Cardiol doi: 10.1016/j.yjmcc.2013.11.015 contributor: fullname: Shinde – volume: 120 start-page: 49 year: 2009 ident: 2019091808530319400_cvx152-B45 article-title: The role of circulating mesenchymal progenitor cells, fibrocytes, in promoting pulmonary fibrosis publication-title: Trans Am Clin Climatol Assoc contributor: fullname: Strieter – volume: 3 start-page: 349 year: 2002 ident: 2019091808530319400_cvx152-B48 article-title: Myofibroblasts and mechano-regulation of connective tissue remodelling publication-title: Nat Rev Mol Cell Biol doi: 10.1038/nrm809 contributor: fullname: Tomasek
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Snippet	Abstract Aims Cardiac fibroblasts (CFs) are considered the principal regulators of cardiac fibrosis. Factors that influence CF activity are difficult to... Cardiac fibroblasts (CFs) are considered the principal regulators of cardiac fibrosis. Factors that influence CF activity are difficult to determine. When... AimsCardiac fibroblasts (CFs) are considered the principal regulators of cardiac fibrosis. Factors that influence CF activity are difficult to determine. When...
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SubjectTerms	Actins - metabolism Angiotensin II - metabolism Animals Biomarkers - metabolism Cell Proliferation - drug effects Collagen - metabolism Dogs Fibroblasts - drug effects Fibroblasts - metabolism Fibroblasts - pathology Fibrosis Humans Mice, Transgenic Myocardium - metabolism Myocardium - pathology Original Phenotype Physical Stimulation Time Factors Tissue Culture Techniques Transforming Growth Factor beta - pharmacology Vimentin - metabolism
Title	Investigation of cardiac fibroblasts using myocardial slices
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