Mapping replication origin sequences in eukaryotic chromosomes

Recent advances in genome-sequencing technology have led to the complete mapping of DNA replication initiation sites in the human genome. This thorough origin mapping facilitates understanding of the relationship between replication initiation events, transcription, and chromatin modifications, and...

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Published in:	Current protocols in cell biology Vol. 65; p. 22.20.1
Main Authors:	Fu, Haiqing, Besnard, Emilie, Desprat, Romain, Ryan, Michael, Kahli, Malik, Lemaitre, Jean-Marc, Aladjem, Mirit I
Format:	Journal Article
Language:	English
Published:	United States 01-12-2014
Subjects:	Animals Chromosome Mapping - methods Chromosomes, Human - genetics DNA Replication Humans Replication Origin DNA replication next-generation sequencing (NGS) replication origins
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Abstract	Recent advances in genome-sequencing technology have led to the complete mapping of DNA replication initiation sites in the human genome. This thorough origin mapping facilitates understanding of the relationship between replication initiation events, transcription, and chromatin modifications, and allows the characterization of consensus sequences of potential replication origins. This unit provides a detailed protocol for isolation and sequence analysis of nascent DNA strands. Two variations of the protocol based on non-overlapping assumptions are described below, addressing potential bias issues for whole-genome analyses.
AbstractList	Recent advances in genome-sequencing technology have led to the complete mapping of DNA replication initiation sites in the human genome. This thorough origin mapping facilitates understanding of the relationship between replication initiation events, transcription, and chromatin modifications, and allows the characterization of consensus sequences of potential replication origins. This unit provides a detailed protocol for isolation and sequence analysis of nascent DNA strands. Two variations of the protocol based on non-overlapping assumptions are described below, addressing potential bias issues for whole-genome analyses.
Author	Ryan, Michael Desprat, Romain Besnard, Emilie Fu, Haiqing Kahli, Malik Lemaitre, Jean-Marc Aladjem, Mirit I
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BackLink	https://www.ncbi.nlm.nih.gov/pubmed/25447077$$D View this record in MEDLINE/PubMed
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CitedBy_id	crossref_primary_10_1016_j_cell_2021_06_025 crossref_primary_10_1186_s13072_016_0067_3 crossref_primary_10_1038_s41596_018_0081_y crossref_primary_10_1186_s13059_020_01983_8 crossref_primary_10_1038_s41467_021_23835_0 crossref_primary_10_3390_cells12222630 crossref_primary_10_1002_bies_202300051 crossref_primary_10_1186_s12915_023_01527_z crossref_primary_10_1016_j_bbamcr_2023_119546 crossref_primary_10_1016_j_celrep_2016_11_041 crossref_primary_10_1126_science_abj5502 crossref_primary_10_1016_j_molcel_2018_01_012 crossref_primary_10_1093_nar_gkac299
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Copyright	Copyright © 2014 John Wiley & Sons, Inc.
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References	21813623 - Genome Res. 2011 Nov;21(11):1822-32 9417033 - Science. 1998 Jan 2;279(5347):95-8 20711191 - Nat Struct Mol Biol. 2010 Sep;17(9):1079-87 24787348 - PLoS Genet. 2014 May;10(5):e1004319 22751019 - Nat Struct Mol Biol. 2012 Aug;19(8):837-44
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SubjectTerms	Animals Chromosome Mapping - methods Chromosomes, Human - genetics DNA Replication Humans Replication Origin
Title	Mapping replication origin sequences in eukaryotic chromosomes
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