Mapping replication origin sequences in eukaryotic chromosomes

Recent advances in genome-sequencing technology have led to the complete mapping of DNA replication initiation sites in the human genome. This thorough origin mapping facilitates understanding of the relationship between replication initiation events, transcription, and chromatin modifications, and...

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Bibliographic Details
Published in:	Current protocols in cell biology Vol. 65; p. 22.20.1
Main Authors:	Fu, Haiqing, Besnard, Emilie, Desprat, Romain, Ryan, Michael, Kahli, Malik, Lemaitre, Jean-Marc, Aladjem, Mirit I
Format:	Journal Article
Language:	English
Published:	United States 01-12-2014
Subjects:	Animals Chromosome Mapping - methods Chromosomes, Human - genetics DNA Replication Humans Replication Origin DNA replication next-generation sequencing (NGS) replication origins
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Summary:	Recent advances in genome-sequencing technology have led to the complete mapping of DNA replication initiation sites in the human genome. This thorough origin mapping facilitates understanding of the relationship between replication initiation events, transcription, and chromatin modifications, and allows the characterization of consensus sequences of potential replication origins. This unit provides a detailed protocol for isolation and sequence analysis of nascent DNA strands. Two variations of the protocol based on non-overlapping assumptions are described below, addressing potential bias issues for whole-genome analyses.
ISSN:	1934-2616
DOI:	10.1002/0471143030.cb2220s65