Genome sizes of the entomopathogenic nematodes Steinernema carpocapsae and Heterorhabditis bacteriophora (Nematoda:Rhabditida)

There is currently no information on the genome size and complexity in the entomoparasitic nematodes Steinernema and Heterorhabditis. DNA reassociation kinetics were used to determine the genome size and complexity in 2 species: Steinernema carpocapsae and Heterorhabditis bacteriophora. C0t curves d...

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Bibliographic Details
Published in:	Parasitology Vol. 114 ( Pt 5); p. 497
Main Authors:	Grenier, E, Catzeflis, F M, Abad, P
Format:	Journal Article
Language:	English
Published:	England 01-05-1997
Subjects:	Animals DNA, Helminth - chemistry DNA, Helminth - genetics Genome Kinetics Molecular Weight Nucleic Acid Renaturation Repetitive Sequences, Nucleic Acid Rhabditoidea - genetics
Online Access:	Get more information
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Summary:	There is currently no information on the genome size and complexity in the entomoparasitic nematodes Steinernema and Heterorhabditis. DNA reassociation kinetics were used to determine the genome size and complexity in 2 species: Steinernema carpocapsae and Heterorhabditis bacteriophora. C0t curves derived from renaturation kinetics of denatured DNA indicate that the genomes of these entomoparasitic rhabditids are different both in size and complexity. Genome sizes were estimated at 2.3 x 10(8) bp for S. carpocapsae and 3.9 x 10(7) bp for H. bacteriophora and repetitive DNA contents were found to represent 39% and 51% of these respective genomes.
ISSN:	0031-1820
DOI:	10.1017/S0031182096008736