Pronuclear abnormalities and cytoskeletal organization during assisted fertilization in a patient with multifollicular ovarian response

• Published in: Journal of assisted reproduction and genetics Vol. 19; no. 3; pp. 152 - 157
• Main Authors: RAWE, V. Y, KOPELMAN, S, NODAR, F. N, OLMEDO, S. Brugo, CHILLIK, C. F
• Format: Journal Article
• Language: English
• Published: New York, NY Kluwer/Plenum 01-03-2002; Springer Nature B.V; Kluwer Academic Publishers-Plenum Publishers
• Subjects: Acetylation; Adult; Biological and medical sciences; Chromatin - ultrastructure; Cryopreservation; Cytoskeleton - chemistry; Cytoskeleton - ultrastructure; Embryo Transfer; Embryos; Female; Fertilization in Vitro; Fundamental and applied biological sciences. Psychology; Humans; In vitro fertilization; Infant, Newborn; Laboratories; Male; Mammalian reproduction. General aspects; Meiosis; Microscopy; Microscopy, Fluorescence; Microscopy, Phase-Contrast; Monoclonal antibodies; Ovarian Hyperstimulation Syndrome - etiology; Ovaries; Ovulation Induction - adverse effects; Patients; Pregnancy; Protein Processing, Post-Translational; Sperm; Sperm Injections, Intracytoplasmic; Sperm Tail - chemistry; Sperm Tail - ultrastructure; Spermatozoa; Tubulin - analysis; Tubulin - chemistry; Ultrasonic imaging; Uterus; Vertebrates: reproduction; Zygote - chemistry; Zygote - pathology; Human; Chromatin; Tubulin; Zygote; Assisted procreation; Cytoskeleton; Female; Microtubule; Germinal cell; Fecundation; Immunofluorescence; Oocyte
• ISSN: 1058-0468; 1573-7330
• DOI: 10.1023/A:1014745006944

To analyze the distribution of a tubulins and acetylated alpha tubulins and the chromatin configuration in abnormally fertilized zygotes from a patient with a multifollicular ovarian response after in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI).
Immunofluorescence and phase contrast microscopy was performed in abnormally fertilized zygotes.
After phase contrast microscopy analysis, immunofluorescence staining was performed in 20 oocytes that developed > or = 3 pronuclei (PN) and karyomeres after IVF-ICSI. Around 80% of the abnormal zygotes from IVF were the consequence of monospermic fertilizations. Retention of the second polar body (PB) and the presumptive split of > or =1 PN within the cytoplasm were the main events present in most oocytes after IVF-ICSI.
Fluorescence labeling of selected sperm and oocyte components affords a unique view of abnormal fertilized zygotes. Surprisingly, anomalies detected after IVF-ICSI showed similar etiologies in this special group of zygotes.