Pseudomonas sp. strain KC represents a new genomovar within Pseudomonas stutzeri

• Published in: International journal of systematic and evolutionary microbiology Vol. 51; no. 6; pp. 2013 - 2019
• Main Authors: Sepulveda-Torres, LC, Zhou, J, Guasp, C, Lalucat, J, Knaebel, D, Plank, JL, Criddle, CS
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01-11-2001; Society for General Microbiology
• Subjects: Bacteriology; Biodegradation, Environmental; Biological and medical sciences; Carbon Tetrachloride - metabolism; DNA Gyrase - genetics; DNA, Ribosomal - genetics; Fresh Water - microbiology; Fundamental and applied biological sciences. Psychology; Inositol - metabolism; Microbiology; Molecular Sequence Data; Motility, taxis; Nitrites - metabolism; Nucleic Acid Hybridization; Phenotype; Phylogeny; Polymerase Chain Reaction; Pseudomonas - classification; Pseudomonas - genetics; Pseudomonas - metabolism; Pyridines - chemistry; Pyridines - metabolism; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Systematics; Pseudomonadales; Volatile compound; Chloroform; Taxonomy; Pollution control; Carbon tetrachloride; Carbon; Pseudomonas stutzeri; Aquifers; Strain; Organochlorine compounds; Chelating agent; Denitrification; Bacteria; Pseudomonadaceae; Isolate; Isolation; Ecological recovery; Bioremediation; Pseudomonas putida
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/00207713-51-6-2013

LC Sepulveda-Torres, J Zhou, C Guasp, J Lalucat, D Knaebel, JL Plank and CS Criddle
Department of Microbiology and National Science Foundation Center for Microbial Ecology, Michigan State University, East Lansing, MI 48823, USA
Pseudomonas sp. strain KC (=ATCC 55595=DSM 7136) is a denitrifying aquifer
isolate that produces and secretes pyridine-2,6-bis(thiocarboxylate)
(PDTC), a chelating agent that fortuitously transforms carbon tetrachloride
without producing chloroform. Although KC has been used successfully for
full-scale bioremediation of carbon tetrachloride, its taxonomy has proven
difficult to resolve, as it retains properties of both Pseudomonas stutzeri
and Pseudomonas putida. In the present work, a polyphasic approach was used
to conclude that strain KC represents a new genomovar (genomovar 9) within
the species P. stutzeri.