Initiation factors IF1 and IF2 synergistically remove peptidyl-tRNAs with short polypeptides from the P-site of translating Escherichia coli ribosomes

A novel function of initiation factors IF1 and IF2 in Escherichia coli translation has been identified. It is shown that these factors efficiently catalyse dissociation of peptidyl-tRNAs with polypeptides of different length from the P-site of E. coli ribosomes, and that the simultaneous presence of...

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Bibliographic Details
Published in:	Journal of molecular biology Vol. 281; no. 2; pp. 241 - 252
Main Authors:	Karimi, Reza, Pavlov, Michael Yu, Heurgué-Hamard, Valérie, Buckingham, Richard H., Ehrenberg, Måns
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 14-08-1998
Subjects:	Carboxylic Ester Hydrolases - metabolism drop-off Escherichia coli Escherichia coli - genetics Escherichia coli - growth & development Guanosine Triphosphate - physiology initiation factors Oligopeptides - metabolism Peptide Initiation Factors - metabolism Peptide Initiation Factors - pharmacology peptidyl-tRNAs Prokaryotic Initiation Factor-2 Protein Biosynthesis - physiology ribosomal P-site Ribosomes - metabolism RNA, Transfer, Amino Acyl - metabolism RNA, Transfer, Met - metabolism translation ribosomal P-site DTE Pth PMSF drop-off translation PEP PK peptidyl-tRNAs initiation factors MK ERRORS SUPPRESSION PROTEIN-SYNTHESIS DISSOCIATION PURIFICATION GENE-EXPRESSION A-SITE TRANSFER-RNA HYDROLASE BINDING
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