Strategy to enhance transgene expression in proximity of amyloid plaques in a mouse model of Alzheimer's disease

• Published in: Theranostics Vol. 9; no. 26; pp. 8127 - 8137
• Main Authors: Weber-Adrian, Danielle, Kofoed, Rikke Hahn, Chan, Josephine Wing Yee, Silburt, Joseph, Noroozian, Zeinab, Kügler, Sebastian, Hynynen, Kullervo, Aubert, Isabelle
• Format: Journal Article
• Language: English
• Published: Australia Ivyspring International Publisher Pty Ltd 01-01-2019; Ivyspring International Publisher
• Subjects: Alzheimer Disease - pathology; Alzheimer's disease; Amyloid beta-Peptides - metabolism; Animals; Astrocytes - metabolism; Blood-brain barrier; Brain; Clinical trials; Disease Models, Animal; Gene Expression; Gene therapy; Gene Transfer Techniques; Glial Fibrillary Acidic Protein - genetics; Glial Fibrillary Acidic Protein - metabolism; Liver - metabolism; Mice; Mice, Transgenic; Plaque, Amyloid - metabolism; Plaque, Amyloid - pathology; Promoter Regions, Genetic; Proteins; Research Paper; Spinal cord; Transgenes; Ultrasonic imaging; amyloid-beta peptides; astrocytes; TgCRND8 mice; focused ultrasound; gene expression
• ISSN: 1838-7640; 1838-7640
• DOI: 10.7150/thno.36718

Gene therapy can be designed to efficiently counter pathological features characteristic of neurodegenerative disorders. Here, we took advantage of the glial fibrillary acidic protein (GFAP) promoter to preferentially enhance transgene expression near plaques composed of amyloid-beta peptides (Aβ), a hallmark of Alzheimer's disease (AD), in the TgCRND8 mouse model of amyloidosis.
The delivery of intravenously injected recombinant adeno-associated virus mosaic serotype 1/2 (rAAV1/2) to the cortex and hippocampus of TgCRND8 mice was facilitated using transcranial MRI-guided focused ultrasound in combination with microbubbles (MRIgFUS), which transiently and locally increases the permeability of the blood-brain barrier (BBB). rAAV1/2 expression of the reporter green fluorescent protein (GFP) under a GFAP promoter was compared to GFP expression driven by the constitutive human beta actin (HBA) promoter.
MRIgFUS targeting the cortex and hippocampus facilitated the entry of rAAV1/2 and GFP expression under the GFAP promoter was localized to GFAP-positive astrocytes. Adjacent to Aβ plaques where GFAP is upregulated, the volume, surface area, and fluorescence intensity of the transgene GFP were greater in rAAV1/2-GFAP-GFP compared to rAAV1/2-HBA-GFP treated animals. In peripheral organs, GFP expression was particularly strong in the liver, irrespective of the promoter.
The GFAP promoter enhanced transgene expression in proximity of Aβ plaques in the brain of TgCRND8 mice, and it also resulted in significant expression in the liver. Future gene therapies for neurological disorders could benefit from using a GFAP promoter to regulate transgene expression in response to disease-induced astrocytic reactivity.