Passive haemagglutination test for human neurocysticercosis immunodiagnosis. I: Standardization and evaluation of the passive haemagglutination test for the detection of anti-Cysticercus cellulosae antibodies

• Published in: Revista do Instituto de Medicina Tropical de São Paulo Vol. 30; no. 1; pp. 51 - 56
• Main Authors: UEDA, M, DIAS CAMARGO, E, JOSE VAZ, A, CARVALHO DE SOUZA, A. M, FIGUEIREDO, R. M, VINICIUS DA SILVA, M
• Format: Journal Article
• Language: English
• Published: São Paulo Instituto de Medicina Tropical de São Paulo 01-02-1988; Instituto de Medicina Tropical; Universidade de São Paulo (USP)
• Subjects: Animals; Antibodies, Helminth - cerebrospinal fluid; Antigens, Helminth - standards; Biological and medical sciences; Central Nervous System Diseases - cerebrospinal fluid; Cerebrospinal fluid; Cysticercosis; Cysticercosis - cerebrospinal fluid; Cysticercus - immunology; Diseases caused by cestodes; Helminthic diseases; Hemagglutination Tests - methods; Humans; Infectious diseases; Medical sciences; Nervous system involvement in other diseases. Miscellaneous; Neurocysticercosis; Neurology; Parasitic diseases; Passive haemagglutination test; Taenia - immunology; TROPICAL MEDICINE; South America; Human; Brain; Nervous system diseases; Cysticercosis; Passive hemagglutination test; Antibody; Immunoreagent; Standardization; Helminthiasis; Immunological method; Cestod disease; Infection; America; Brazil; Diagnosis; Passive haemagglutination test; Neurocysticercosis; Cerebrospinal fluid
• ISSN: 0036-4665; 1678-9946; 1678-9946; 0036-4665
• DOI: 10.1590/S0036-46651988000100009

A passive haemagglutination test (PHA) for human neurocysticercosis was standardized and evaluated for the detection of specific antibodies to Cysticercus cellulosae in cerebrospinal fluid (CSF). For the assay, formaldehyde-treated group O Rh-human red cells coated with the cysticerci crude total saline extract (TS) antigen were employed. A total of 115 CSF samples from patients with neurocysticercosis was analysed, of these 94 presented reactivity, corresponding to 81.7% sensitivity, in which confidence limit of 95% probability (CL95%) ranged from 74.5% to 88.9%. Eighty-nine CSF samples derived from individuals of control group presented as nonreactive in 94.4% (CL95% from 89.6% to 99.2%). The positive and negative predictive values were 1.4% and 99.9%, respectively, considering the mean rate of that this assay provide a rapid, highly reproducible, and moderately sensitive mean of detecting specific antibodies in CSF samples.
Foi padronizada e avaliada a reação de hemaglutinação passiva (RHA) para pesquisa de anticorpos específicos, anti-Cysticercus cellulosae, no líquido cefalorraquiano (LCR). Foram utilizadas hemácias humanas O Rh-formolizadas e sensibilizadas com extrato antigênico salino total de cisticercos, ainda pouco estudado. De 115 amostras estudadas de LCR de pacientes com neurocisticercose, 94 foram reagentes, resultando em 81,7% de sensibilidade, com intervalo de confiança de 95% de probabilidade (IC95%) abrangendo de 74,5% e 88,9%. Também foram ensaiadas 89 amostras de LCR de indivíduos do grupo controle, sendo tão reagentes em 94,4%, com IC95%, de 89,6% a 99,2%. Os valores preditivos positivo e negativo obtidos para a RHA foram, respectivamente, de 1,4% e 99,9%, considerando a prevalência média de neurocisticercose na América Latina de 0,1%. Os resultados indicam que a RHA como um método simples, altamente reprodutível e moderadamente sensível para a detecção de anticorpos específicos no LCR, porém apropriados para a triagem de infectados.