Chemoselective One-Step Alkylation and Isolation of Thiophosphorylated Cdk2 Substrates in the Presence of Native Cysteine

The elucidation of signalling pathways relies heavily upon the identification of protein kinase substrates. Recent investigations have demonstrated the efficacy of chemical genetics using ATP analogues and modified protein kinases for specific substrate labelling. Here we combine N⁶-(cyclohexyl)ATPγ...

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Bibliographic Details
Published in:	Chembiochem : a European journal of chemical biology Vol. 12; no. 4; pp. 633 - 640
Main Authors:	Lee, Sarah E, Elphick, Lucy M, Kramer, Holger B, Jones, Alexandra M.E, Child, Emma S, Anderson, Alexandra A, Bonnac, Laurent, Suwaki, Natsuko, Kessler, Benedikt M, Gouverneur, Véronique, Mann, David J
Format:	Journal Article
Language:	English
Published:	Weinheim Wiley-VCH Verlag 07-03-2011 WILEY-VCH Verlag WILEY‐VCH Verlag
Subjects:	Alkylation ATP cdk2 chemical genetics Chlorides - chemistry cyclin Cyclin-Dependent Kinase 2 - chemistry Cyclin-Dependent Kinase 2 - genetics Cysteine - chemistry Electrophoresis, Polyacrylamide Gel Hydrogen-Ion Concentration kinases Peptides - chemistry Peptides - genetics Phosphorus Compounds - chemistry Signal Transduction substrate isolation Substrate Specificity thiophosphorylation
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