Chemoselective One-Step Alkylation and Isolation of Thiophosphorylated Cdk2 Substrates in the Presence of Native Cysteine
The elucidation of signalling pathways relies heavily upon the identification of protein kinase substrates. Recent investigations have demonstrated the efficacy of chemical genetics using ATP analogues and modified protein kinases for specific substrate labelling. Here we combine N⁶-(cyclohexyl)ATPγ...
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| Published in: | Chembiochem : a European journal of chemical biology Vol. 12; no. 4; pp. 633 - 640 |
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| Main Authors: | , , , , , , , , , , |
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| Language: | English |
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Weinheim
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| Abstract | The elucidation of signalling pathways relies heavily upon the identification of protein kinase substrates. Recent investigations have demonstrated the efficacy of chemical genetics using ATP analogues and modified protein kinases for specific substrate labelling. Here we combine N⁶-(cyclohexyl)ATPγS with an analogue-sensitive cdk2 variant to thiophosphorylate its substrates and demonstrate a pH-dependent, chemoselective, one-step alkylation to facilitate the detection or isolation of thiophosphorylated peptides. |
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| AbstractList | The elucidation of signalling pathways relies heavily upon the identification of protein kinase substrates. Recent investigations have demonstrated the efficacy of chemical genetics using ATP analogues and modified protein kinases for specific substrate labelling. Here we combine
N
6
‐(cyclohexyl)ATPγS with an analogue‐sensitive cdk2 variant to thiophosphorylate its substrates and demonstrate a pH‐dependent, chemoselective, one‐step alkylation to facilitate the detection or isolation of thiophosphorylated peptides. The elucidation of signalling pathways relies heavily upon the identification of protein kinase substrates. Recent investigations have demonstrated the efficacy of chemical genetics using ATP analogues and modified protein kinases for specific substrate labelling. Here we combine N(6) -(cyclohexyl)ATPγS with an analogue-sensitive cdk2 variant to thiophosphorylate its substrates and demonstrate a pH-dependent, chemoselective, one-step alkylation to facilitate the detection or isolation of thiophosphorylated peptides. The elucidation of signalling pathways relies heavily upon the identification of protein kinase substrates. Recent investigations have demonstrated the efficacy of chemical genetics using ATP analogues and modified protein kinases for specific substrate labelling. Here we combine N⁶-(cyclohexyl)ATPγS with an analogue-sensitive cdk2 variant to thiophosphorylate its substrates and demonstrate a pH-dependent, chemoselective, one-step alkylation to facilitate the detection or isolation of thiophosphorylated peptides. The elucidation of signalling pathways relies heavily upon the identification of protein kinase substrates. Recent investigations have demonstrated the efficacy of chemical genetics using ATP analogues and modified protein kinases for specific substrate labelling. Here we combine N6‐(cyclohexyl)ATPγS with an analogue‐sensitive cdk2 variant to thiophosphorylate its substrates and demonstrate a pH‐dependent, chemoselective, one‐step alkylation to facilitate the detection or isolation of thiophosphorylated peptides. It's a kinase magic: The identification of protein substrates is hampered by the large number of kinases active in cells at a given time. Here we use a chemical genetic approach to specifically label the substrates of an analogue‐sensitive kinase with thiophosphate and use pH‐dependent S‐alkylation to selectively recover thiophosphorylated peptides. |
| Author | Anderson, Alexandra A. Suwaki, Natsuko Kessler, Benedikt M. Elphick, Lucy M. Jones, Alexandra M. E. Kramer, Holger B. Gouverneur, Véronique Mann, David J. Bonnac, Laurent Child, Emma S. Lee, Sarah E. |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/21305681$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1038/ncb813 10.1074/jbc.272.8.4759 10.1021/pr060608s 10.1146/annurev.bi.54.070185.002055 10.1002/qsar.200640051 10.1074/mcp.M300039-MCP200 10.1021/cb700027u 10.1016/S0955-0674(02)00317-4 10.1073/pnas.0708966105 10.1038/nbt1076 10.1126/science.1075762 10.1021/ol006055n 10.1016/0968-0004(92)90010-7 10.1002/cbic.200900052 10.1016/S0960-9822(98)70198-8 10.1042/BJ20070797 |
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| Copyright | Copyright © 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
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| References | E. P. Go, W. Uritboonthai, J. V. Apon, S. A. Trauger, A. Nordstrom, G. O'Maille, S. M. Brittain, E. C. Peters, G. Siuzdak, J. Proteome Res. 2007, 6, 1492-1499. L. M. Elphick, S. E. Lee, V. Gouverneur, D. J. Mann, ACS Chem. Biol. 2007, 2, 299-314. D. Coverley, H. Laman, R. A. Laskey, Nat. Cell Biol. 2002, 4, 523-528. F. Eckstein, Annu. Rev. Biochem. 1985, 54, 367-402. J. Bain, L. Plater, M. Eliott, N. Shpiro, C. J. Hastie, H. McLauchlan, I. Klevernic, J. S. Arthur, D. R. Alessi, P. Cohen, Biochem. J. 2007, 408, 297-315. R. M. Phan, C. D. Poulter, Org. Lett. 2000, 2, 2287-2289. T. Kupke, F. Gotz, J. Biol. Chem. 1997, 272, 4759-4762. S. W. Kwon, S. C. Kim, J. Jaunbergs, J. R. Falck, Y. Zhao, Mol. Cell. Proteomics 2003, 2, 242-247. P. Cohen, Trends Biochem. Sci. 1992, 17, 408-413. G. Manning, D. B. Whyte, R. Martinez, T. Hunter, S. Sudarsanam, Science 2002, 298, 1912-1934. A. C. Bishop, K. Shah, Y. Liu, L. Witucki, C. Kung, K. M. Shokat, Curr. Biol. 1998, 8, 257-266. S. M. Brittain, S. B. Ficarro, A. Brock, E. C. Peters, Nat. Biotechnol. 2005, 23, 463-468. J. D. Blethrow, J. S. Glavy, D. O. Morgan, K. M. Shokat, Proc. Natl. Acad. Sci. USA 2008, 105, 1442-1447. K. M. Specht, K. M. Shokat, Curr. Opin. Cell Biol. 2002, 14, 155-159. L. M. Elphick, S. E. Lee, E. S. Child, A. Prasad, C. Pignocchi, S. Thibaudeau, A. A. Anderson, L. Bonnac, V. Gouverneur, D. J. Mann, ChemBioChem 2009, 10, 1519-1526. S. Dandapani, QSAR Comb. Sci. 2006, 25, 681-688. 2002; 14 2009; 10 1997; 272 2002; 298 2006; 25 1992; 17 2003; 2 2007; 6 2002; 4 2008; 105 2000; 2 2007; 2 2007; 408 1985; 54 2005; 23 1998; 8 e_1_2_6_8_2 e_1_2_6_7_2 e_1_2_6_9_2 e_1_2_6_4_2 e_1_2_6_3_2 e_1_2_6_6_2 e_1_2_6_5_2 e_1_2_6_12_2 e_1_2_6_13_2 e_1_2_6_2_2 e_1_2_6_10_2 e_1_2_6_1_2 e_1_2_6_11_2 e_1_2_6_16_2 e_1_2_6_14_2 e_1_2_6_15_2 |
| References_xml | – volume: 23 start-page: 463 year: 2005 end-page: 468 publication-title: Nat. Biotechnol. – volume: 8 start-page: 257 year: 1998 end-page: 266 publication-title: Curr. Biol. – volume: 25 start-page: 681 year: 2006 end-page: 688 publication-title: QSAR Comb. Sci. – volume: 4 start-page: 523 year: 2002 end-page: 528 publication-title: Nat. Cell Biol. – volume: 298 start-page: 1912 year: 2002 end-page: 1934 publication-title: Science – volume: 14 start-page: 155 year: 2002 end-page: 159 publication-title: Curr. Opin. Cell Biol. – volume: 105 start-page: 1442 year: 2008 end-page: 1447 publication-title: Proc. Natl. Acad. Sci. USA – volume: 54 start-page: 367 year: 1985 end-page: 402 publication-title: Annu. Rev. Biochem. – volume: 272 start-page: 4759 year: 1997 end-page: 4762 publication-title: J. Biol. Chem. – volume: 2 start-page: 299 year: 2007 end-page: 314 publication-title: ACS Chem. Biol. – volume: 6 start-page: 1492 year: 2007 end-page: 1499 publication-title: J. Proteome Res. – volume: 2 start-page: 2287 year: 2000 end-page: 2289 publication-title: Org. Lett. – volume: 2 start-page: 242 year: 2003 end-page: 247 publication-title: Mol. Cell. Proteomics – volume: 408 start-page: 297 year: 2007 end-page: 315 publication-title: Biochem. J. – volume: 10 start-page: 1519 year: 2009 end-page: 1526 publication-title: ChemBioChem – volume: 17 start-page: 408 year: 1992 end-page: 413 publication-title: Trends Biochem. Sci. – ident: e_1_2_6_16_2 doi: 10.1038/ncb813 – ident: e_1_2_6_10_2 doi: 10.1074/jbc.272.8.4759 – ident: e_1_2_6_14_2 doi: 10.1021/pr060608s – ident: e_1_2_6_6_2 doi: 10.1146/annurev.bi.54.070185.002055 – ident: e_1_2_6_13_2 doi: 10.1002/qsar.200640051 – ident: e_1_2_6_11_2 doi: 10.1074/mcp.M300039-MCP200 – ident: e_1_2_6_3_2 doi: 10.1021/cb700027u – ident: e_1_2_6_4_2 doi: 10.1016/S0955-0674(02)00317-4 – ident: e_1_2_6_7_2 doi: 10.1073/pnas.0708966105 – ident: e_1_2_6_12_2 doi: 10.1038/nbt1076 – ident: e_1_2_6_2_2 doi: 10.1126/science.1075762 – ident: e_1_2_6_9_2 doi: 10.1021/ol006055n – ident: e_1_2_6_1_2 doi: 10.1016/0968-0004(92)90010-7 – ident: e_1_2_6_8_2 doi: 10.1002/cbic.200900052 – ident: e_1_2_6_5_2 doi: 10.1016/S0960-9822(98)70198-8 – ident: e_1_2_6_15_2 doi: 10.1042/BJ20070797 |
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| SubjectTerms | Alkylation ATP cdk2 chemical genetics Chlorides - chemistry cyclin Cyclin-Dependent Kinase 2 - chemistry Cyclin-Dependent Kinase 2 - genetics Cysteine - chemistry Electrophoresis, Polyacrylamide Gel Hydrogen-Ion Concentration kinases Peptides - chemistry Peptides - genetics Phosphorus Compounds - chemistry Signal Transduction substrate isolation Substrate Specificity thiophosphorylation |
| Title | Chemoselective One-Step Alkylation and Isolation of Thiophosphorylated Cdk2 Substrates in the Presence of Native Cysteine |
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