Effect of Solution Conformation on Antibody Recognition of a Protein Core Epitope from Gastrointestinal Mucin (MUC2)

• Published in: Archives of biochemistry and biophysics Vol. 361; no. 1; pp. 65 - 74
• Main Authors: Uray, Katalin, Kajtár, Judit, Vass, Elemér, Price, Michael R., Hollósi, Miklós, Hudecz, Ferenc
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-01-1999
• Subjects: Amino Acid Sequence; Antibodies, Monoclonal - metabolism; Binding Sites, Antibody; Circular Dichroism; Epitopes - immunology; FT-IR; Humans; immunochemistry; Intestinal Mucosa - chemistry; Intestinal Mucosa - immunology; Models, Molecular; Molecular Sequence Data; MUC2 mucin; Mucin-2; Mucins - chemistry; Mucins - immunology; Mucins - metabolism; Peptide Fragments - chemical synthesis; Peptide Fragments - chemistry; Peptide Fragments - immunology; Protein Conformation; Protein Structure, Secondary; Repetitive Sequences, Amino Acid - immunology; secondary structure of synthetic peptides; Solutions; Spectroscopy, Fourier Transform Infrared; MUC2 mucin; CD; secondary structure of synthetic peptides; FT-IR; immunochemistry
• ISSN: 0003-9861; 1096-0384
• DOI: 10.1006/abbi.1998.0955

Antibody recognition of the tandem repeat unit of MUC2 glycoprotein was investigated. To clarify the role of secondary structure, the immunoreactivity and conformation of overlapping and truncated peptides were investigated. For this several MUC2 peptides have been synthesized and their secondary structure has been analyzed by circular dichroism and Fourier transform infrared spectroscopical methods. For the binding studies a MUC2 mucin protein core-specific monoclonal antibody was used in competition RIA experiments. The minimal size peptide functioning as epitope was peptide18PTGTQ22. Within the immunodominant13TPTPTPTGTQTPTT26region we found that all peptides recognized by the 996 monoclonal antibody adopted β-turns secondary structure. Peptides15TPTPTGTQ22and16PTPTGTQ22, containing the most prominent β-turn(s), had the strongest immunoreactivity. It was also observed that peptides with Pro on their N-termini (16PTPTGTQ22,18PTGTQ22) adopt a different type of β-turn in TFE than peptides with Thr at their N-terminal. Based on the antibody binding, molecular dynamics calculations, and secondary structure analysis, we propose a model for the epitope structure of the MUC2 mucin tandem repeat.