Fractionation of a hydrocolloid emulsifier reclaimed from winery waste
•A natural emulsifier is extracted from wine processing waste.•The natural extract is fractionated via preparative size exclusion chromatography.•The natural emulsifier consists of 4 main molecular populations of different MW.•The chemical composition and surface activity of each population is asses...
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Published in: | Food chemistry Vol. 301; p. 125259 |
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15-12-2019
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Abstract | •A natural emulsifier is extracted from wine processing waste.•The natural extract is fractionated via preparative size exclusion chromatography.•The natural emulsifier consists of 4 main molecular populations of different MW.•The chemical composition and surface activity of each population is assessed.•This information is used as to model the collective action of the composite extract.
Complex hydrocolloids have been isolated and fractionated using a consecutive elution process, starting from winery waste. These extracts consist mainly of polysaccharidic populations and of smaller protein molecules and they exhibit emulsifying, thickening and texture-modifying activity. This work is a systematic study of these individual populations, as fractionated with preparative size exclusion chromatography (Prep-SEC) in terms of their chemical identity, surface properties, and emulsification behavior. The fractions have been characterized via SEC-MALLS, FTIR, DLS, zeta potential, and interfacial tension measurements. The results highlight the antagonistic and synergistic effects of the individual components of the above-mentioned complex natural material (winery waste extract) towards its emulsifying behavior, and provide a model for the kinetics of the evolution of a Pickering interfacial layer. |
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AbstractList | •A natural emulsifier is extracted from wine processing waste.•The natural extract is fractionated via preparative size exclusion chromatography.•The natural emulsifier consists of 4 main molecular populations of different MW.•The chemical composition and surface activity of each population is assessed.•This information is used as to model the collective action of the composite extract.
Complex hydrocolloids have been isolated and fractionated using a consecutive elution process, starting from winery waste. These extracts consist mainly of polysaccharidic populations and of smaller protein molecules and they exhibit emulsifying, thickening and texture-modifying activity. This work is a systematic study of these individual populations, as fractionated with preparative size exclusion chromatography (Prep-SEC) in terms of their chemical identity, surface properties, and emulsification behavior. The fractions have been characterized via SEC-MALLS, FTIR, DLS, zeta potential, and interfacial tension measurements. The results highlight the antagonistic and synergistic effects of the individual components of the above-mentioned complex natural material (winery waste extract) towards its emulsifying behavior, and provide a model for the kinetics of the evolution of a Pickering interfacial layer. Complex hydrocolloids have been isolated and fractionated using a consecutive elution process, starting from winery waste. These extracts consist mainly of polysaccharidic populations and of smaller protein molecules and they exhibit emulsifying, thickening and texture-modifying activity. This work is a systematic study of these individual populations, as fractionated with preparative size exclusion chromatography (Prep-SEC) in terms of their chemical identity, surface properties, and emulsification behavior. The fractions have been characterized via SEC-MALLS, FTIR, DLS, zeta potential, and interfacial tension measurements. The results highlight the antagonistic and synergistic effects of the individual components of the above-mentioned complex natural material (winery waste extract) towards its emulsifying behavior, and provide a model for the kinetics of the evolution of a Pickering interfacial layer. |
ArticleNumber | 125259 |
Author | Pavlou, Alexandros Georgiou, Despoina Panayiotou, Costas Kalogianni, Eleni P. Ritzoulis, Christos |
Author_xml | – sequence: 1 givenname: Alexandros surname: Pavlou fullname: Pavlou, Alexandros organization: Department of Food Science and Technology, International Hellenic University, Sindos Campus, 57400 Thessaloniki, Greece – sequence: 2 givenname: Costas surname: Panayiotou fullname: Panayiotou, Costas organization: Department of Chemical Engineering, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece – sequence: 3 givenname: Eleni P. surname: Kalogianni fullname: Kalogianni, Eleni P. organization: Department of Food Science and Technology, International Hellenic University, Sindos Campus, 57400 Thessaloniki, Greece – sequence: 4 givenname: Despoina surname: Georgiou fullname: Georgiou, Despoina organization: Department of Food Science and Technology, International Hellenic University, Sindos Campus, 57400 Thessaloniki, Greece – sequence: 5 givenname: Christos orcidid: 0000-0002-9664-8455 surname: Ritzoulis fullname: Ritzoulis, Christos email: critzou@food.teithe.gr organization: Department of Food Science and Technology, International Hellenic University, Sindos Campus, 57400 Thessaloniki, Greece |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/31376691$$D View this record in MEDLINE/PubMed |
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Cites_doi | 10.1080/10408699891274354 10.1016/j.chroma.2004.12.002 10.1002/bmc.1130070204 10.1016/S0165-022X(03)00065-4 10.1007/s00216-010-4221-7 10.1016/j.foodhyd.2015.02.029 10.1016/0003-2697(90)90627-L 10.1016/j.foodhyd.2015.08.026 10.1007/s12649-015-9476-y |
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Keywords | UV Winery waste MALLS MS DLS MW AIS bp Preparative size exclusion chromatography SEC AR Emulsion FTIR O/W Interfacial tension RI Emulsifiers Pickering stabilization GPC SLS GC HPLC ATR |
Language | English |
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References | Fedarko, Termine, Robey (b0010) 1990; 188 Filotheou, Ritzoulis, Avgidou, Kalogianni, Pavlou, Panayiotou (b0015) 2015; 48 Schmitt, Sanchez, Desobry-Banon, Hardy (b0040) 1998; 38 Xiong, Zhang, Wang (b0050) 2005; 1063 McClements (b0025) 2015 Pavlou, Ritzoulis, Filotheou, Panayiotou (b0035) 2016; 7 Cosgrove (b0005) 2010 Temenouga, Charitidis, Avgidou, Karayannakidis, Dimopoulou, Kalogianni, Ritzoulis (b0045) 2016; 52 Parker, Finnie, Raouf, Ryder, Campbell, Tsai, Rhodes (b0030) 1993; 7 Gaborieau, Castignolles (b0020) 2011; 399 Zhang, Zhang, Cheung, Dong (b0055) 2003; 56 Filotheou (10.1016/j.foodchem.2019.125259_b0015) 2015; 48 Cosgrove (10.1016/j.foodchem.2019.125259_b0005) 2010 Gaborieau (10.1016/j.foodchem.2019.125259_b0020) 2011; 399 Zhang (10.1016/j.foodchem.2019.125259_b0055) 2003; 56 McClements (10.1016/j.foodchem.2019.125259_b0025) 2015 Xiong (10.1016/j.foodchem.2019.125259_b0050) 2005; 1063 Pavlou (10.1016/j.foodchem.2019.125259_b0035) 2016; 7 Temenouga (10.1016/j.foodchem.2019.125259_b0045) 2016; 52 Fedarko (10.1016/j.foodchem.2019.125259_b0010) 1990; 188 Parker (10.1016/j.foodchem.2019.125259_b0030) 1993; 7 Schmitt (10.1016/j.foodchem.2019.125259_b0040) 1998; 38 |
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contributor: fullname: Schmitt – volume: 1063 start-page: 71 issue: 1–2 year: 2005 ident: 10.1016/j.foodchem.2019.125259_b0050 article-title: Polymer fractionation using chromatographic column packed with novel regenerated cellulose beads modified with silane publication-title: Journal of Chromatography A doi: 10.1016/j.chroma.2004.12.002 contributor: fullname: Xiong – volume: 7 start-page: 68 issue: 2 year: 1993 ident: 10.1016/j.foodchem.2019.125259_b0030 article-title: High performance gel filtration using monodisperse highly cross-linked agarose as a one-step system for mucin purification publication-title: Biomedical Chromatography doi: 10.1002/bmc.1130070204 contributor: fullname: Parker – volume: 56 start-page: 281 issue: 1–3 year: 2003 ident: 10.1016/j.foodchem.2019.125259_b0055 article-title: Fractionation and characterization of a Polysaccharide from the sclerotia of Pleurotus tuber-regium by preparative size-exclusion chromatography publication-title: Journal of Biochemical 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volume: 52 start-page: 972 year: 2016 ident: 10.1016/j.foodchem.2019.125259_b0045 article-title: Novel emulsifiers as products from internal Maillard reactions in okra hydrocolloid mucilage publication-title: Food Hydrocolloids doi: 10.1016/j.foodhyd.2015.08.026 contributor: fullname: Temenouga – volume: 7 start-page: 533 issue: 3 year: 2016 ident: 10.1016/j.foodchem.2019.125259_b0035 article-title: Emulsifiers extracted from winery waste publication-title: Waste and Biomass Valorization doi: 10.1007/s12649-015-9476-y contributor: fullname: Pavlou – year: 2010 ident: 10.1016/j.foodchem.2019.125259_b0005 contributor: fullname: Cosgrove |
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Snippet | •A natural emulsifier is extracted from wine processing waste.•The natural extract is fractionated via preparative size exclusion chromatography.•The natural... Complex hydrocolloids have been isolated and fractionated using a consecutive elution process, starting from winery waste. These extracts consist mainly of... |
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SubjectTerms | Emulsifiers Emulsion Interfacial tension Pickering stabilization Preparative size exclusion chromatography Winery waste |
Title | Fractionation of a hydrocolloid emulsifier reclaimed from winery waste |
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