Identification and quantification of phenolic compounds in berry skin, pulp, and seeds in 13 grapevine varieties grown in Serbia

• Published in: Food chemistry Vol. 211; pp. 243 - 252
• Main Authors: Pantelić, Milica M., Dabić Zagorac, Dragana Č., Davidović, Sonja M., Todić, Slavica R., Bešlić, Zoran S., Gašić, Uroš M., Tešić, Živoslav Lj, Natić, Maja M.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 15-11-2016
• Subjects: Anthocyanins; Anthocyanins - analysis; Antioxidant activity; Catechin - analogs & derivatives; Catechin - analysis; Chromatography, High Pressure Liquid; Flavonoids - analysis; Flavonols - analysis; Food Analysis - methods; Free Radical Scavengers; Fruit - chemistry; Grape; LC/MS; Mass Spectrometry; PCA; Phenolic profile; Phenols - analysis; Plant Extracts - chemistry; Polyphenols - analysis; Seeds - chemistry; Serbia; Vitis - chemistry; LC/MS; Anthocyanins; Phenolic profile; Grape; Antioxidant activity; PCA
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2016.05.051

•Polyphenolic profile of 13 grape varieties was determined.•Quantification of the polyphenols was done using UHPLC-DAD MS/MS.•Ellagic acid was determined in the seeds of all the examined varieties.•A total of twenty anthocyanins was identified by UHPLC-MS/MS OrbiTrap.•Malvidin 3,5-O-dihexoside was identified in all studied red grapevine skins.
Phenolic profile of 13 grapevine varieties was determined, with respect to three different parts of berries: skin, seed and pulp. Identification and quantification of the phenols was done using ultra-high performance liquid chromatography (UHPLC) coupled with a diode array detector and a triple-quadrupole mass spectrometer. The most abundant phenols in grape seeds were found to be flavan-3-ols, most of which are gallocatechin gallate and catechin. The skins were characterized mostly by flavonols, i.e. quercetin and myricetin. Characterization of anthocyanins in the berry skin by UHPLC coupled with Linear Trap Quadrupole and OrbiTrap mass analyzer revealed a total of twenty derivatives of malvidin, delphinidin, petunidin, cyanidin, and peonidin. To the extent of our knowledge this is the first work that shows the presence of malvidin 3,5-O-dihexoside in the berry skin of ‘Merlot’, ‘Cabernet Franc’, ‘Shiraz’ ‘Sangiovese’, ‘Pinot Noir’ and ‘Prokupac’, untypical for Vitis vinifera Linneo species.