Comparison of different nucleic acid preparation methods to improve specific HIV-1 RNA isolation for viral load testing on dried blood spots

•m2000sp (Abbott) did not improve RNA recovery from DBS.•DNase treatments increased false negative results (VF≥1000 copies/mL).•Free virus elution increased false negative results (VF≥1000 copies/mL).•Viral load assays on DBS need improvement of nucleic acid preparation methods. In resource-limited...

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Bibliographic Details
Published in:	Journal of virological methods Vol. 251; pp. 75 - 79
Main Authors:	Guichet, Emilande, Serrano, Laetitia, Laurent, Christian, Eymard-Duvernay, Sabrina, Kuaban, Christopher, Vidal, Laurent, Delaporte, Eric, Ngole, Eitel Mpoudi, Ayouba, Ahidjo, Peeters, Martine
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-01-2018 Elsevier
Subjects:	Dried blood spot False Negative Reactions False Positive Reactions HIV Infections - virology HIV-1 HIV-1 - isolation & purification Humans Life Sciences Microbiology and Parasitology Plasma - virology Resource limited countries RNA extraction RNA, Viral - analysis RNA, Viral - isolation & purification Santé publique et épidémiologie Specimen Handling - methods Viral load Viral Load - methods Virology HIV-1 RNA extraction Resource limited countries Viral load Dried blood spot
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